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Recommendations for Pen-based Oral Fluid Collection in Growing Pigs

17 July 2014

For pigs familiar with a sampling rope, 20 minutes is sufficient time to obtain an effective oral fluid sample for the diagnosis of a number of diseases, according to new Iowa State University research.

Researchers at the College of Veterinary Medicine at Iowa State University have investigated how long a sampling rope should be left in place to achieve the best representation of the pigs in the pen.

In a paper in the Journal of Swine Health and Production, first-name author, Drew White and co-authors report their work on developing sampling guidelines by observing pigs during oral-fluid sample collection in commercial herds.

They concluded that pigs with previous oral-fluid collection experience ('trained') should be allowed 20 minutes access to the rope. Pigs with no prior experience ('untrained') should be allowed 60 minutes. One collection is enough to train pigs.

Assays have been described for oral-fluid specimens using antibody- and PCR-based assays for a variety of pathogens, including porcine reproductive and respiratory syndrome (PRRS) virus, influenza A virus, porcine circovirus type (PCV) and African swine fever virus.


White D., M. Rotolo, C. Olsen, C. Wang, J. Prickett, A. Kittawornrat, Y. Panyasing, R. Main, C. Rademacher, M. Hoogland and J.J. Zimmerman. 2014. Recommendations for pen-based oral-fluid collection in growing pigs. J. Swine Health Prod. 22(3):138–141.

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July 2014

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