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International PRRS and PCVAD Symposium 2013

30 September 2014

Proceedings of the 2013 International Porcine Reproductive and Respiratory Syndrome (PRRS) Symposium and PCVAD (PCV2), held at the China National Conference Center in Beijing, China from 20 to 22 May 2013.

Click on the link at the end to access the full proceedings and individual abstracts.

  • EUROPRRS: Still paving the way? - T. Ait-Ali. p30
  • Fostera™ PRRS: Modulation of interferon-alpha and induction of protective immunity two weeks following vaccination - J.G. Calvert et al. p31
  • Porcine Reproductive and Respiratory Syndrome (PRRSV) Diagnostics in the United States: Past, Present and Future - J. Christopher-Hennings. p32
  • Vaccine vectors based on Coronavirus genomes to protect against PRRSV and SARS-CoV - L. Enjuanes et al. p33
  • The PRRSV replicase: structure, function and implications - Y. Fang et al. p34
  • Principles of controlling PCV2 – the importance of controlling viremia - M.L. Linatoc p35
  • Reflection of sow serology in piglet litters oral fluids – a sow farm surveillance field study - C.K. Goodell et al. p36
  • Genetics of host resistance to PRRSV infection: Progress of the PRRS Host Genetics Consortium - J.K. Lunney et al. p37
  • An update on PRRS immunology - E. Mateu. p38
  • Breeding for PRRS resistance - G. McLaren and N. Deeb. p39
  • Control of PRRS on farm and regional level - R. Morrison. p40
  • Global Research on PRRS – development of STAR-IDAZ PRRS network - A. Morrow. p41
  • Immunological interactions of PRRSV and pigs - M.P. Murtaugh et al. p42
  • The pathogenesis of porcine reproductive and respiratory syndrome (PRRS) is fully determined by the intriguing interplay of PRRSV with monocytic cells - H. Nauwynck et al. p43
  • Luminex® application for PCV2 and PRRSV diagnosis - T. Opriessnig. p44
  • The role of host genetics in vaccine development - R.R.R. Rowland and B. Trible. p45
  • Molecular evolution of PRRSV in Europe - T. Stadejek. p46
  • Development and application of a dual-marker vaccine for highly pathogenic Porcine Reproductive and Respiratory Syndrome - G.Z. Tong et al. p47
  • Porcine reproductive and respiratory syndrome situation in China: the past, present, and future - H. Yang. p48
  • Scale-up and transformation of Chinese swine production in the next decade and analysis of high-efficacy hog production strategies - G. Yi. p49
  • Structure and function of PRRSV proteins - D. Yoo et al. p50
  • Towards a better PRRS vaccine: what are the missing links? Z. Wei et al. p51

Epidemiology and Disease Control

  • Feed addition with Tilmicosin reduce PRRSV viral loads in pigs in vivo - M.T. Chiou et al. p52
  • Operating system for gene surveillance of porcine reproductive and respiratory syndrome virus (PRRSV) in Korea - E.J. Choi et al. p53
  • Development of Real Time RTPCR (RRT-PCR) commercial kit for reliable detection and typing of PPRSV - E. Redondo et al. p54
  • PRRSV elimination in a 600 sow one-site system in China - Z.G. Liang et al. p55
  • Evidence that Torque teno sus virus 1 (TTSuV1) is not associated with porcine circovirus associated diseases (PCVAD) - Y.W. Huang et al. p56
  • The establishment of the PRRS detection liquid blocking ELISA method - H. Liu et al. p57
  • Seroprevalence of PRRSV, swine influenza (SIV), and PCV2 in feral pigs - M.A. Kerrigan et al. p58
  • Protective effects against PRRSV infection and boosting effects on PRRSV vaccine of immunostimulator (Barodon®) in pigs - C.H. Kwon et al. p59
  • Application of high molecular Poly-gamma-glutamic acid on Pigs infected with Porcine Reproductive and Respiratory Syndrome virus - J.H. Lee et al. p60
  • Genetic analysis and phylogenetic tree of porcine circovirus type 2 field strains isolated from slaughtered pigs in Korea - M.I. Lee et al. p61
  • Recombination leads to the reemergence of highly pathogenic PRRSV in China - M. Shi and F.C. Leung. p62
  • Comparison of viremia of type II porcine reproductive and respiratory syndrome virus in natural PRRSV infected pigs by zip nucleic acid probes based real-time PCR - C.N. Lin et al. p63
  • Comparison and application of several porcine circovirus type 2 antibody detection kits - Z.H. Zhang et al. p64
  • Prevalence of porcine hokovirus and its co-infection with porcine circovirus 2 in China - S.B. Li et al. p65
  • Three new emerging subgroups of torque teno sus virus (TTSuVs) and co-infection of TTSuV with porcine circovirus type 2 in China - J.B. Liu et al. p66
  • Quantification of PRRSV transmission: effect of pig vaccination - E. Pileri et al. p67
  • The isolation and identification of new subtype of PCV2a - Q. Xiaohuo et al. p68
  • Development of best practices to diagnose Porcine Reproductive and Respiratory Syndrome (PRRS) virus subtypes with high sensitivity in swine - R. Rauh et al. p69
  • Discrimination of Porcine Reproductive and Respiratory Syndrome virus type I and type II by an indirect ELISA - M. Frias et al. p70
  • Detection of porcine reproductive and respiratory syndrome virus (PRRSV) using the light scattering assay (LSA) based on the microfluidic chip - J.Y. Song et al. p71
  • Serosurvey and molecular detection of PRRSV-1 in European wild boars (Sus scrofa) in Lithuania - A. Stankevicius et al. p72
  • Genetic analysis of porcine reproductive and respiratory syndrome virus strains in Japan from 1992 to 2011 - M. Takagi et al. p73
  • Vaccination against PRRS and PCV2 reduced the loss in nursery: a case report - D. Xu et al. p74
  • PRRSV and other co-infecting viruses in PMWS affected pigs - S. Vilcek et al. p75
  • The first detection of PRRSV in wild boars hunted in Central European region, Slovakia - S. Vilcek et al. p76
  • Porcine Reproductive and Respiratory Syndrome in hybrid wild boars, China - J.Q. Wu et al. p77
  • Exploration of large breeding herd with PRRSV serum acclimation - S.Q. Wu et al. p78
  • A potential novel Porcine Reproductive and Respiratory Syndrome virus subgroup emerged in south China based on the genetic analysis of the ORF5 gene from 2007 to 2011 - J.X. Xie et al. p79
  • Study on antiviral activity of four Chinese herbs to Porcine Reproductive and Respiratory Syndrome virus. J.J. He et al. p80
  • Effects of ozone, ultraviolet and disinfectant against Porcine Reproductive and Respiratory Syndrome virus - Y.D. Yoon and W.I. Kim. p81
  • Preparation and immune activities of specific transfer factor against porcine reproductive and respiratory syndrome - H.X. Yuan et al. p82
  • Possible cross-species infection of porcine circoviruses and its control measures - S.L. Zhai et al. p83
  • Preparation of monoclonal antibody against PRRSV Nsp9 gene and establishment of block-ELISA method - Z.Q. Huang et al. p 84
  • The Analysis of Genetic Variation among 19 Nsp2 Genes of PRRSV Isolated from Southwestern China in 2009-2012 - Y.S. Zhou et al. p85

Genetics

  • Autophagy is involved in genetic resistance to PRRSV infection - B. Ding et al. p86
  • Cloning and functional analysis of 5′regulatory region of porcine CYP3A88 gene - C.L. Jiang et al. p87
  • Generation of an infectious clone of SDA2, a highly virulent North American-type isolate of porcine reproductive and respiratory syndrome virus - C.L. Jiang et al. p88
  • Cloning and functional analysis of 5′regulatory region of porcine USP18 gene - Y.P. Li et al. p89
  • Porcine circovirus type 2 (PCV2): genetic variation and newly emerging genotypes in China - L.J. Guo et al. p90
  • Analysis of synonymous codon usage in Porcine Reproductive and Respiratory Syndrome virus - Y.S. Liu et al. p91
  • Antiviral activities of the porcine IFIT family genes against PRRSV - X. Zhou et al. p92

Vaccines and Immunity

  • PRRS modified live vaccine adjuvanted with Montanide™ Gel01 ST provides enhanced protection against homologous PRRSV infection in swine - A.J. Galliher-Beckley et al. p93
  • Distribution of PRRSV and PCV2 in sera and tissues and pulmonary findings after PCV2 vaccination in field pigs - C.C. Chang et al. p94
  • The effects on distribution of pathogens, reproductive performance of sows and growth performance of weaned pigs after attenuated live PRRSV vaccination in sows - C.C. Chang and L.C. Yang. p95
  • Evaluation of a DNA vaccine candidate co-expressing GP3 and GP5 of PRRSV with interferon alpha/gamma in immediate and long-lasting protection against highly pathogenic PRRSV challenge - Y.J. Du et al. p96
  • The immunization of pigs with a Gp3 subunit vaccine elicits specific antibody response but does not protect against PRRSV infection after challenge - M. García Durán et al. p97
  • Development and validation of porcine reproductive and respiratory syndrome virus specific neutralizing antibody detection assay in pig oral fluid samples - K. Ouyang et al. p98
  • Characterisation of the T cell response induced by infection with subtype 1 or 3 European porcine reproductive and respiratory syndrome viruses - H. Mokhtar et al. p99
  • Development of a multiplex fluorescent microsphere immunoassay for diagnosis of porcine respiratory disease complex - S.R. Lawson et al. p100
  • Evaluation of the efficacy of an attenuated live vaccine against highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) in young pigs - X. Leng et al. p101
  • Virulence evaluation of a modified live highly pathogenic porcine reproductive and respiratory syndrome virus vaccine by serial passages in pigs - X. Leng et al. p102
  • Changes of different lymphocyte contents in peripheral blood from pigs co-infected with porcine circovirus type 2 and porcine parvovirus - H.Y. Li et al. p103
  • Peptide nanofiber hydrogel: A novel adjuvant for Porcine Reproductive and Respiratory Syndrome virus modified live vaccine - X. Li et al. p104
  • Attenuation of interferon antagonizing function of PRRSV by targeted mutations in a highly conserved motif of nsp1beta protein - Y.H. Li et al. p105
  • Comparative respiratory pathogenicity and dynamic distribution of highly pathogenic porcine reproductive and respiratory syndrome virus and its attenuated vaccine strain in piglets - C. Liu. p106
  • Construction and biological characteristics of a recombinant porcine circovirus type 2 expressing the VP1 neutralizing epitope of foot-and-mouth disease virus - F.Y. Zhang et al. p107
  • Enhanced protective immune response to PCV2 subunit vaccine by co-administration of recombinant porcine IFN-gamma in mice - Y.P. Wang et al. p108
  • Immunity induced by an experimental vaccine based on the recombinant PRRSV GP5 protein without ADE epitope expressed in adenovirus in pigs - M. Yang et al. p109
  • Modulation of swine interleukin-4 to induce virus-specific immune responses elicited by modified live porcine reproductive and respiratory syndrome virus (PRRSV) vaccine - J. Peng et al. p110
  • Construction and immunogenicity of DNA vaccine coexpressing GP3 and GP5 proteins of European-type Porcine Reproductive and Respiratory Syndrome virus - J.Q. Ren et al. p111
  • Inhibition of porcine circovirus type 2 replication by cap protein gene DNA vaccine in mice - S. Sylla et al. p112
  • Preliminary study of recombinant Bordetella Bronchiseptica expressing cap protein of Porcine Circovirus type 2 as a candidate vaccine against porcine Circovirus disease - S. Jin et al. p113
  • Isolation of the European genotype porcine reproductive and respiratory syndrome virus (PRRSV) in Kazakhstan - K.K. Tabynov et al. p114
  • Effect of vaccination with Ingelvac Circoflex® on fattening performance in a farm in north China - A. Wang et al. p115
  • Construction of recombinant PRRSV expressing granulocytemacrophage colony-stimulating factor - L.X. Yu et al. p116
  • Evidence for a broadly neutralizing antibody response to PRRSV - B.R. Trible et al. p117
  • Host recognition of the structural form of the PCV2 capsid protein results in protective immunity or immunopathogenesis - B.R. Trible and R.R.R. Rowland. p118
  • The comparison of immune efficacy between combined PCV2, MH vaccine inactivated and the corresponding single vaccines - G.H. Wang et al. p119
  • Identification of a B-cell epitope in the glycosylated protein 3 of highly pathogenic porcine reproductive and respiratory syndrome virus HuN4 strain - Y. Sun et al. p120
  • Anti-idiotypic antibody vaccine against highly pathogenic PRRSV infection - C. Zhang et al. p121
  • The nonstructural protein 1 papain-like cysteine protease was not necessary for porcine reproductive and respiratory syndrome virus nonstructural protein 1 to inhibit interferon-beta function - X.B. Shi et al. p122
  • Toll-like receptor ligands enhance the protective effects of vaccination against porcine reproductive and respiratory syndrome virus in swine - L.L. Zhang et al. p123
  • The changes of the lymphocyte subpopulations in peripheral blood of the pigs inoculated with highly pathogenic PRRSV GD wild and vaccine type - W.L. Zhang et al. p124

Viral Heterogeneity and Evolution

  • Investigation of recombination in Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) - R.J. Chand et al. p125
  • A novel isolate with deletion in GP3 gene of Porcine Reproductive and Respiratory Syndrome virus from the mideast of China - B.C. Fan et al. p126
  • swIFN-beta promotes genetic mutation of porcine reproductive and respiratory syndrome virus in Marc-145 - D.S. He et al. p127
  • Experimental infection of pigs with two east European variants of Type 1 PRRSV - C.K. Hjulsage et al. p128
  • Fast and robust methods for full genome sequencing of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Type 1 and Type 2 - L.K. Kvisgaard et al. p129
  • The diversity of Porcine Reproductive and Respiratory Syndrome virus type 1 and 2 in Denmark - L.K. Kvisgaard et al. p130
  • The evidence of porcine respiratory and reproductive syndrome virus evolved under pressure from deaminases - C.L. Leng et al. p131
  • Genomic sequencing and genetic variation analysis of highly pathogenic porcine reproductive and respiratory syndrome virus - C. Liu et al. p132
  • Recombination events are common in genotype 1 and 2 PRRS virus worldwide and may generate mosaic viruses - G.E. Martin-Valls et al. p133
  • Evolution dynamics of highly pathogenic type 2 PRRSV: analysis of envelope protein coding genes - V.G. Nguyen et al. p134
  • Phylogentic analysis and molecular characteristics of five variant Chinese field isolates of PRRSV - Z.F. Pei et al. p136
  • A cell culture-derived deletion in the nsp2 region of a North American PRRSV overlaps with a unique nsp2 deletion reported in a Chinese PRRSV isolate - R. Ransburgh et al. p137
  • A novel strain of PCV2 isolated from vaccinated pigs suffering PCVAD causes 40% mortality in CDCD pigs in the presence of PPV - A.W. Suddith and R. Hesse. p138
  • A monoclonal antibody against the N of a HP-PRRS vaccine strain(HuN4-F112) could not recognize its virulent strain (HuN4-F5) not due to their amino acid sequence difference - Q. Wang et al. p139
  • Molecular basis associated with the pathogenicity of a genotype 1 PRRSV isolate - X.C. Wang et al. p140
  • Phylogenetic analysis and molecular characteristics of 17 porcine reproductive and respiratory syndrome virus isolates in South China from 2010-2011 - M.Z. Zhang et al. p141

Virus Replication-Protein Structure-Function

  • Non-structural protein 1-mediated interferon modulation is a common strategy for immune evasion in arteriviruses - M. Han et al. p142
  • Suppression of type I interferon production mediated by PRRS virus non-structural protein 1-beta - M. Han et al. p143
  • Construction and biological characterization of recombinant porcine circovirus type 2 expressing the V5 epitope tag - L.P. Huang et al. p144
  • Identification of one critical amino acid that determines a conformational neutralizing epitope in the capsid protein of porcine circovirus type 2 - L.P. Huang et al. p145
  • Mutation in a conserved motif of porcine circovirus type 2 capsid protein reduces capsid protein expression, hampers colocalization between capsid and replication proteins and impacts viral fitness - L.P. Huang et al. p146
  • Identification of porcine reproductive and respiratory syndrome virus ORF1a-encoded replicase proteins in virusinfected cells - Y.H. Li et al. p147
  • Antigenic epitope mapping using the monoclonal antibody against the recombinant Rep’ protein of porcine circovirus type 2 - D. Liu et al. p148
  • Characterization of two chimeric viruses by exchanging ORF2 gene of porcine circovirus type 1 (PCV1) and type 2 (PCV2) - C.M. Liu et al. p149
  • Natural recombinant mutants, resulting from replacement of an open reading frame 2 (ORF2)-containing genomic fragment of porcine circovirus (PCV) genotype 2b with that of genotype 2a, showed significantly enhanced viral replication and altered antigenicity in vitro - L.J. Guo et al. p150
  • Discovery and characterization of the involvement of a cellular membrane fusion protein in PRRSV replication - J.A. Hicks et al. p151
  • Amino acid mutations in the capsid protein produce novel porcine circovirus type 2 neutralizing epitopes - J.B. Liu et al. p152
  • RNA interference targeting nucleocapsid protein (N) inhibits porcine reproductive and respiratory syndrome virus replication in Marc-145 cells - M. Yang et al. p153
  • Functional analysis of ORF4 protein encoded by porcine circovirus type 2 - N. Zhou et al. p154
  • ORF4 protein: a newly discovered virus protein in PCV2 replication - J.L. He et al. p155
  • Co-infection study between porcine reproductive and respiratory syndrome virus and a bacteria led to the discovery of a possible new bacteria antiviral - C. Provost et al. p156
  • Impact of co-infection by porcine circovirus (PCV) and porcine reproductive and respiratory syndrome virus (PRRSV) in porcine pulmonary epithelial cell line - C. Provost et al. p157
  • The correlation of cyclin A expression level with porcine circovirus type 2 propagation efficiency - Q.H. Tang et al. p158
  • Dissection for the structure and function of a 5'-proximal prominent stem-loop in Porcine Reproductive and Respiratory Syndrome virus - F. Gao et al. p159
  • Signal peptide cleavage from GP5 of PRRSV: Only a minor subfraction of molecules retains the decoy epitope, a presumed molecular cause for viral persistence - B. Thaa et al. p160
  • Non-structural protein 2 of the porcine reproductive and respiratory syndrome (PRRS) virus: a crucial protein in viral pathogenesis, immunity and diagnosis - F.X. Wang et al. p161
  • Role of non-structural protein 2 in the regulation of the replication of the porcine reproductive and respiratory syndrome virus in MARC-145 cells: effect of gene silencing and over expression - F.X. Wang et al. p162
  • The effect of the position of N-linked glycosylation sites within hypervariable region of GP5 on virus neutralization - Z.Z. Wei et al. p163
  • The role of antibody in PRRSV infection PAM - Q.Y. Yang et al. p164
  • Regulation of host cell gene expression and cell cycle progression by nsp11 of PRRS virus - Y. Sun et al. p165
  • Concurrent highly pathogenic porcine reproductive and respiratory syndrome virus infection accelerates Haemophilus parasuis infection in conventional pigs - J. Yu et al. p166
  • Mutagenesis analysis of porcine reproductive and respiratory syndrome virus nonstructural protein 7 - M.Z. Zhang et al. p167

Virus-Host Interaction

  • The host miR-26a inhibits porcine reproductive and respiratory syndrome virus replication by activating innate antiviral immunity - C.W. Chen et al. p168
  • The application of deep sequencing for the analysis of mutations within hypervariable regions of PRRSV - N. Chen et al. p169
  • The effect of knockdown of Nonmuscle myosin II-A on Porcine Reproductive and Respiratory Syndrome Virus infection - J. Gao et al. p170
  • MicroRNA-181 suppresses PRRSV infection by targeting its receptor CD163 - L. Gao et al. p171
  • Nonstructural proteins 1beta and 11 play an important role in differential TNF-alpha production-induced by different PRRSV strains - Q. He et al. p172
  • Using high throughput proteomics to understand the functional interaction of PRRSV and the nucleocapsid protein with the host cell and comparison with the coronavirus infectious bronchitis virus - H.Y. Chen et al. p173
  • Fetal preservation and virus concentration following type 2 PRRSV infection in pregnant gilts - A. Ladinig et al. p174
  • PRRS RNA concentration in serum does not predict clinical severity in pregnant gilts - A. Ladinig et al. p175
  • Temporal changes in white blood cell counts and PBMC subsets following type 2 PRRSV in pregnant gilts - A. Ladinig et al. p176
  • A porcine circovirus type 2 (PCV2) mutant with 234 amino acids in capsid protein showed more virulence in vivo, compared with classical PCV2a/b strains. L.J. Guo et al. p177
  • The role of Hsp70 in the replication of porcine circovirus type 2 in vitro - J. Liu et al. p178
  • Porcine reproductive and respiratory syndrome virus (PRRSV)-mediated NF-κB activation is dependent on TLR2, TLR4 and MAPK signaling pathway - R.Luo et al. p179
  • Quantitative mitochondrial proteomics by isobaric tags for relative and absolute quantification (iTRAQ) labeling in PRRSV infected pulmonary alveolar macrophages - R. Luo et al. p180
  • PRRSV infection change Bax/Bcl-2 ratio in primary porcine alveolar macrophages - Y. Mu et al. p181
  • Porcine, murine and human sialoadhesin: portals for PRRSV entry into target cells - W. Van Breedam et al. p182
  • PRRSV infection induces Sn expression on local endometrial and placental CD163-positive cells - U.U. Karniychuk et al. p183
  • Phenotypic modulation and cytokine profiles of antigen presenting cells by European subtype 1 and 3 porcine reproductive and respiratory syndrome virus strains in vitro and in vivo - E. Weesendorp et al. p184
  • Genome-wide analysis of marker genes related to antiviral regulation in PRRSV-infected porcine macrophages at different activation statuses - Y. Sang et al. p185
  • In vivo impact of deoxynivalenol naturally contaminated feed on porcine reproductive and respiratory virus infection - C. Savard et al. p186
  • The susceptibility of porcine myeloid cells to Porcine Reproductive and Respiratory Syndrome virus - H. Singleton et al. p187
  • PRRSV type 1 can induce aggravation of infection with a mild virulent Actinobacillus serotype 2 strain - N. Stockhofe-Zurwieden et al. p188
  • Identification of differentially expressed proteins in porcine alveolar macrophages infected with virulent/attenuated strains of porcine reproductive and respiratory syndrome virus - J.P. Zhu et al. p189
  • Porcine SAMHD1 blocks HPPRRSV replication in Marc-145 cells - S. Yang et al. p190
  • Reduction of PRRSV infection in Marc-145 cells that express the exogenous C-terminal domain of non-muscle myosin heavy chain II-A - R. Wei et al. p191
  • Experimental infection and comparative genomic analysis of a highly pathogenic PRRSV-HBR strain at different passage levels - Y.W. Wei et al. p192
  • Synergistic effects of sequential infection with highly pathogenic porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 in piglets - P.H. Fan et al. p193
  • Swine immunoglobulin lambda-like polypeptide 5, an adaptor of RIG-1 and MDA5, regulates type I Interferon production and PRRSV replication through interaction with PRRSV non-structural proteins - Q.F. Liu et al. p194
  • Heme oxygenase 1 inhibition of HP-PRRSV infection in Marc-145 cell lines - A. Zhang et al. p195
  • USP18 restricts PRRSV growth through alteration of nuclear translocation of NF-κB p65 and p50 in MARC-145 cells - D. Xu et al. p196
  • Induction of ROS generation and NF-kappa B activation in MARC-145 cells by a novel Porcine Reproductive and Respiratory Syndrome virus isolated in the southwest of China - Y. Yan et al. p197
  • Nonstructural proteins 4 and 10 of highly pathogenic PRRSV induce apoptosis in virus-infected MARC-145 cells - S.Z. Yuan et al. p198
  • Porcine reproductive and respiratory syndrome virus activates inflammasomes of porcine alveolar macrophages via its small envelope protein E - K. Zhang et al. p199
  • Non-muscle myosin heavy chain II-A is a novel cellular receptor for PRRSV - J. Gao et al. p200

Further Reading

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Find out more about PRRS by clicking here.

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