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Bulletin No. 19 - Fall 2004
VirologyPMWS-PCV2-PCVD-PDNS
SANCHEZ RE, MEERTS P, NAUWYNCK HJ, ELLIS JA, PENSAERT MB
Characteristics of porcine circovirus-2 replication in lymphoid organs of pigs inoculated in late gestation or postnatally and possible relation to clinical and pathological outcome of infection
J Vet Diagn Invest, 16, 2004, 175-185
Both late-term fetuses in utero and one-day-old cesarean derived piglets were inoculated with PCV2 in an attempt to reproduce PMWS and PMWS-like lesions. Piglets were either euthanized immediately at harvest or sacrificed sequentially and monitored for clinical signs. In each case, blood and tissue samples from lymphoid organs were collected and virus isolation and titration were performed in order to correlate the level of virus replication with clinical signs, histological lesions and serological response. PCV2 infected cells were identified by double-immunofluorescence labeling using several specific cell markers. Clinical signs were not observed in any of the piglets suggesting that high levels of PCV2 replication and severe lymphoid lesions are not necessarily associated with wasting disease which is in disagreement with some previous studies. Cells of the monocyte-macrophage lineage were confirmed the main target cells of PCV2. However, infected cells carrying lymphocyte-related markers were also detected in a few pigs without it being possible to state for sure that these cells were lymphocytes. Six out of the 26 piglets included in this study had high virus titers (104.5-5.7 TCID50/g) with infiltrating monocytic cells, and to a lesser extent cells carrying lymphocyte-related markers, as the main infected cell populations while in pigs with lower virus titers (less than 104.5 TCID50/g) cells of the monocyte-macrophage lineage were those primarily infected.
