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Bulletin No. 19 - Fall 2004
VirologyPMWS-PCV2-PCVD-PDNS
SIPOS W, DUVIGNEAU JC, WILLHEIM M, SCHILCHER F, HARTL RT, HOFBAUER G, EXEL B, PIETSCHMANN P, SCHMOLL F
Systemic cytokine profile in feeder pigs suffering from natural postweaning multisystemic wasting syndrome (PMWS) as determined by semiquantitative RT-PCR and flow cytometric intracellular cytokine detection
Veterinary Immunology and Immunopathology, 97, 2004, 25-37
The blood cell and cytokine profile of 14-week-old PMWS-naturally-affected pigs was compared to that of clinically healthy pen-mates. In PMWS-affected pigs, total leukocytes and platelets were increased whereas lymphocytes were only slightly decreased but this could be ascribed to the fact that the animals being 14-week-old were then coming out of the clinical stage of the disease (4-14 weeks of age). At the transcriptional level, an increase in IL-1a and IL-10 mRNA was observed in PMWS-affected pigs but the difference was not significant when compared to reference pigs, whereas IL-2 and IL-2Ra mRNA was found notably decreased even if the results of flow cytometry revealed a significant increase of IL-2 at the protein level and thus an increased capacity of the remaining lymphocytes to produce IL-2 upon in vitro stimulation. No difference was observed between diseased and healthy animals for IL-12 and TNF-a and only a slight increase in IFN-? was detected in the diseased pigs. As a virus-induced disease, PMWS should lead to a cellular immune response characterized either by a Th1 driven cytokine response pattern or a cytokine pattern indicative of T cell immunosuppression. The results of this study contrast with this hypothesis.
