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Bulletin No. 19 - Fall 2004
VirologyPMWS-PCV2-PCVD-PDNS
MCNAIR I, MARSHALL M, MCNEILLY F, BOTNER A, LADEKJAER-MIKKELSEN AS, VINCENT I, HERRMANN B, SANCHEZ R, RHODES C
Interlaboratory testing of porcine sera for antibodies to porcine circovirus type 2
J Vet Diagn Invest, 16, 2004, 164-166
Various types of assays and fixatives are currently available for the detection and the titration of PCV2 antibodies in swine sera. Five laboratories across Europe and Canada were given the same panel of 20 swine sera and requested to carry out their routine assays. The analysis of the results revealed that immunoperoxydase monolayer assay (IPMA) provided higher titers than did indirect immunofluorescence assay (IFA) and that paraformaldehyde provided higher titers than did acetone or ethyl alcohol. These variations clearly demonstrates that the investigation of PCV2 calls for standardized procedures.
