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Bulletin No. 19 - Fall 2004
VirologyPMWS-PCV2-PCVD-PDNS
FENAUX M, OPRIESSNIG T, HALBUR PG, ELVINGER F, MENG XJ
A chimeric porcine circovirus (PCV) with the immunogenic capsid gene of the pathogenic PCV type 2 (PCV2) cloned into the genomic backbone of the nonpathogenic PCV1 induces protective immunity against PCV2 infection in pigs
Journal of Virology, 2004, Vol. 78, Nº12, 6297-6303
In a previous paper, Fenaux et al. reported that they had designed an immunogenic, attenuated chimeric PCV1-2. This follow-up study consisted in inoculating 3 groups of 12 SPF piglets either by intramuscular injection with 200 µg of the chimeric PCV1-2 DNA clone (group 1), by intralymphoid injection with 200 µg of the chimeric PCV1-2 DNA clone (group 2) or by intramuscular injection with 103.5 TCID50 of the chimeric PCV1-2 live virus (group 3), a fourth group of 12 pigs being kept as control. At 42 days post-inoculation all pigs were challenged intranasally and intramuscularly with 2x104.5 TCID50 of a wild-type PCV2. The results of this study showed that protective immunity to wild-type PCV2 was achieved in SPF pigs using attenuated chimeric PCV1-2 live virus or DNA clone inoculated intramuscularly (group 1 and 3).
