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Bulletin No. 19 - Fall 2004
18th IPVS Congress, Hamburg, Germany, 2004PMWS-PCV2-PCVD-PDNS
CAPRIOLI A, MCNEILLY F, MCNAIR I, MCKILLEN J, LAGAN P, ASKIN C, ELLIS J, ALLAN G
Diagnosis of PMWS by PCR: PCR for PCV2 should not be used for diagnosis of PMWS
Proceedings of the 18th IPVS Congress, Hamburg, Germany, 2004 - Volume 1:80
Blood samples, fecal swabs and tonsil swabs were collected in experimentally PCV2-infected pigs and examined by a non-quantitative PCR for PCV2 DNA. A 25 days post-infection the pigs were euthanized and tissue samples were submitted to histopathological examination and immunofluorescence for PCV2 antigen. No correlation could be demonstrated between the results of the non-quantitative PCR and the occurrence of PMWS confirming that PCR for PCV2 is not a suitable tool for diagnosis of PMWS.



