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Bulletin No. 19 - Fall 2004
18th IPVS Congress, Hamburg, Germany, 2004Porcine Reproductive and Respiratory Syndrome (PRRS)
DE WIT JJ, CIA C, BOLINK GJ, DE JONG MF,, DONADEU M
Comparison of 4 ELISAs for detection of antibodies against PRRSV in pig herds with different situations of PRRSV infection
Proceedings of the 18th IPVS Congress, Hamburg, Germany, 2004 - Volume 1:34
The genomic and antigenic variability of PRRSV strains hinders the development of a diagnostic test capable of detecting antibodies directed against all strains of PRRSV. Several ELISAs are available for the detection of PRRSV antibodies but their performance need to be further investigated. Two of the PRRSV antibody ELISAs included in this study were developed to detect PRRSV group specific antibodies whereas the other two were developed to detect either the European strains or the American strains. The sera used in this study were obtained from 441 pigs originating from 5 PRRS-affected farms. The variation in the results obtained with the 4 ELISAs indicates that various factors such as the type of PRRSV strain, the phase of infection or even the test itself, may greatly influence the answer to the test.
