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Bulletin No. 19 - Fall 2004
18th IPVS Congress, Hamburg, Germany, 2004Porcine Reproductive and Respiratory Syndrome (PRRS)
MIELI L, BAUDOUARD M, LEBON E, JOISEL
Qualification and evaluation of a new PRRSV IgM IPMA test
Proceedings of the 18th IPVS Congress, Hamburg, Germany, 2004 - Volume 1:127
Currently, PRRSV antibody determination is based on the detection of IgG. However, such methods have been demonstrated to provide sometimes questionable results. The use of an IgM immunoperoxidase monolayer assay (IPMA) was evaluated with sera from experimentally PRRSV-infected pigs collected at different days post-infection. The results obtained by this technique were similar to those obtained by other previously described IgM detection techniques, i.e. immunofluorescence antibody (IFA) assay. The authors recommend the use of IPMA in association with PCR in order to differentiate between recent infections and stabilized infections as well as between passive immunity and active seroconversion in post-weaning pigs up to 10 weeks.
