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Bulletin No. 19 - Fall 2004
18th IPVS Congress, Hamburg, Germany, 2004Classical Swine Fever
DEWULF J, KOENEN F, MINTIENS K, DENIS P, RIBBENS S, DE KRUIF A
Analytical performance of several laboratory diagnostic techniques on live animals for early detection of a classical swine fever
Proceedings of the 18th IPVS Congress, Hamburg, Germany, 2004 - Volume 1:342
Several assays were evaluated with regards to their ability of detecting truly CSFV-infected pigs: virus isolation in different blood fractions (whole blood, leukocytes, serum, plasma), Ag ELISA, leukocyte count for leukopenia and RT-nPCR. None of them were able to detect all infected pigs. RT-nPCR resulted able to detect the higher proportion of infected animals (98.9%). However RT-nPCR remains an expensive and time-consuming diagnostic technique. Further developments of this technique should allow to ease its use for more complex situations in the near future.
