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Bulletin No. 20 - Winter 2004
BacterologyMycoplasma hyopneumoniae
DUBOSSON CR, CONZELMANN C, MISEREZ R, BOERLIN P, FREY J, ZIMMERMANN W, HANI H, KUHNERT P
Development of two real-time PCR assays for the detection of Mycoplasma hyopneumoniae in clinical samples.
Veterinary Microbiology, 2004, Volume 102, Nº1-2, 55-65
Two real-time-PCR methods of detection of Mycoplasma hyopneumoniae are described. Each one is based on the amplification of one target gene, i.e. a repeated DNA element (REP) and a gene encoding for an ABC transporter protein (ABC) of M. hyopneumoniae. The REP-rtPCR and the ABC-rtPCR were performed on bronchial swabs collected in lungs of swine originating from 16 farms with known sanitary status regarding enzootic pneumonia (11 positive and 5 negative). Both assays were shown 100% specific without any cross reaction with other bacteria or mollicutes. The REP-rtPCR and the ABC-rtPCR were run in parallel in all samples. The ABC rtPCR proved to be highly sensitive (70%-sensitivity) and the use of the REP rtPCR in association with the ABC rtPCR allowed to increase sensitivity which reached 85%. Running these two rtPCR in parallel is thus a reliable means for the detection of M. hyopneumoniae.
