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Bulletin No. 20 - Winter 2004
VirologyPorcine Reproductive and Respiratory Syndrome (PRRS)
PRIETO C, GARCIA C, SIMARRO I, CASTRO JM
Temporal shedding and persistence of porcine reproductive and respiratory syndrome virus in boars.
Veterinary Record, 2004, Volume 154, N?26, 824-827
The experiment consisted in inoculating mature boars with a Spanish PRRSV isolate and monitoring periodically serum and tissue samples as well as body fluids for 37 days for the presence and the amount of PRRSV. One or two boars were euthanized everyday for 10 days and then every 5 or 6 days. Urine, nasal, fecal, prepucial and oropharingeal swabs were collected and assayed. The results of the presence and distribution of PRRSV are summarized in the following table:
| Samples | Fecal samples | Nasal samples | Urine samples | Prepucial samples | Orophar. samples | Lymph nodes | Lungs | Tonsils | Thymus | Peyer's patches |
| Frequency of detection | 30% | 25% | 25% | 25% | 10% | 123/140 | 18/20 | 19/20 | 11/16 | 13/16 |
| Period of detection (DPI) | 7-23 | 3-6 | 3-13 | 4-9 | 5-9 | 2-37 | No positive sample after 17 DPI | |||
PRRSV was shown to be detected more frequently and for a longer period in feces suggesting that the oro-fecal route may play an important role in the transmission of the disease. Moreover, PRRSV was shown to persist in lymph nodes, lungs and tonsils after cessation of viremia.
