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Bulletin No. 21 - Spring 2005
VirologyInternational Society for Animal Hygiene ? Saint-Malo - 2004
FONI E, CHIAPPONI C, GARBARINO C, BARIGAZZI G, MERENDA M
Detection and subtyping of swine influenza virus by RT-PCR and standard methods.
Proceedings of the International Society for Animal Hygiene, 2004, 333-334
The inoculation of embryonated eggs (EEI) and cell cultures (CCI) are the reference tests for SIV infection diagnosis while hemagglutination inhibition (HI) and neuraminidase inhibition (NI) are the techniques usually used to identify swine influenza viruses (SIV) subtypes. EEI and techniques were compared to an RT-PCR based on the matrix (M) gene and HI and NI tests were compared to two multiplex RT-PCR methods. The high sensitivity of the RT-PCR method indicates that it could be used as a screening test on pools of samples before the application of virus isolation through EEI or CCI. The results of subtyping were less convincing since the multiplex RT-PCR method failed several times to identify the tested SIV subtype, in particular in case of co-infection with two subtypes. The use of RT-PCR seems thus more appropriate for SIV detection.
