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Bulletin No. 22 - Summer 2005
VirologyPMWS-PCV2-PCVD-PDNS
CAPRIOLI A, OSTANELLO F, MC NEILLY F, MC NAIRI I, ELLIS J, ALLAN G
Diagnosi di infezione da circovirus suino tipo 2 (PCV2) e della postweaning multisystemic wasting syndrome (PMWS) mediante analisi PCR di campioni di sangue, feci e tamponi tonsillari prelevati da animali vivi [PCR detection of PCV2 in blood, tonsil
Società Italiana di Patologia ed Allevamento dei Suini - Proceedings, 2005, 321-330
Twelve pigs were inoculated with PCV2 at 5 weeks of age. Following infection, blood samples, feces and tonsil swabs were collected every 3 days and submitted to detection of PCV2 by qualitative PCR. At 21 days post-infection, all animals were euthanized and tissue samples were submitted to histopathological examination for PMWS lesions and indirect immunofluorescence for PCV2 antigen. At the end of the study (21 days PI), PCV2 was detected in the blood samples of all animals and in the feces and tonsil swabs of 11 animals. PMWS lesions and PCV2 antigen were present in only four pigs which, in fact, showed a significantly higher number of PCR-positive blood samples than the other pigs. These results confirm that PCV2 DNA can be detected in samples from pigs with or without pathological and virological evidence of PMWS but also that PCR alone is not sufficient to establish a diagnosis of PMWS. However, the fact that PCV2 DNA was detected more frequently in pigs with PMWS-typical lesions suggests that PCV2 DNA detection if associated to PCV2 DNA quantification could be a useful diagnostic tool for PMWS diagnosis in live pigs.
