Swine Bibliography Centre
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Bulletin No. 24 - Spring 2006
VirologyPMWS-PCV2-PCVD-PDNS
MEERTS P, MISINZO G, NAUWYNCK HJ
Interferon-gamma enhances porcine circovirus 2 infection in vitro.
International Conference on Animal Circoviruses and Associated Diseases, European Society for Veterinary Virology, 2005
The objective of this study was to monitor in vitro the effect of some recombinant porcine cytokines (IL-1, IL-6, TNF- α, IFN- α, IL-10 and IFN- γ) on PCV2 infection. PK-15 or 3D4/31 cells were seeded, a dilution series was added to the medium and PCV2 strain Stoon-1010 was inoculated. Thirty-six hours after inoculation, the cells were fixed and stained for PCV2 antigens and the effect of IFN-γ on the fixation and internalisation of PCV2 to its target cells was determined by means of confocal microscopy. The results showed that IFN-γ did enhance viral infection.
