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Bulletin No. 24 - Spring 2006

Virology

PMWS-PCV2-PCVD-PDNS

BLANCHARD P, ROSE N, GRASLAND B, OGER A, EVENO E, MADEC F, JESTIN A
Benefit of PCV2 serological and genomic load profiles on PMWS diagnosis in field cases.
International Conference on Animal Circoviruses and Associated Diseases, European Society for Veterinary Virology, 2005

This study aimed at assessing the serological profile and the genomic load profile in porcine circovirus type 2 (PCV2) infection. The study was carried out in 3 farms affected by postweaning multisystemic wasting sndrome (PMWS), in which pregant sows were studied during pregnancy and at weaning time, and piglets were studied at several time points between 7 and 21 weeks of age. Serological profile was assessed with an Orf2-protein ELISA test and genomic load profile was assessed from DNA extracted from serum, using Wizard SV 96 Genomic DNA purification system. PCV2 genome was quantified by a real-time PCR. The results showed that serological and genomic load profiles were important to determine the PCV2 infection dynamics.


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