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Bulletin No. 24 - Spring 2006
VirologyPMWS-PCV2-PCVD-PDNS
MEERTS P, VAN GUCHT S, COX E, VANDEBOSCH A, NAUWYNCK HJ
Correlation between type of adaptive immune response against porcine circovirus type 2 and level of virus replication.
Viral Immunology, 2005, Volume 18, N°2, 333-341
The aim of this study was to determine which immunological mechanisms lead to the replication of porcine circovirus type 2 (PCV2) in pigs. Sixteen gnotobiotic piglets were inoculated with PCV2 when they were 19-day old. Cyclosporin A (CysA) was then administered to some of those pigs in order to suppress the adaptive immunity and thus to make it possible to assess its effect on the development of PCV2 infection. Several analysis were carried out (lymphoid tissues biopsies, monitoring of clinical signs, identification of PCV2-specific antibodies by use of an immunoperoxydase monolayer assay - IPMA, detection of IFN-γ mRNA by use of real-time reverse transcriptase polymerase chain reaction - RT-PCR, statistical analysis). The results showed that the adaptive immunity could be responsible for the differences observed in PCV2 replication in pigs.
