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Bulletin No. 30 - Summer 2008
BacterologyMycoplasma hyopneumoniae
MAROIS C, LE DEVENDEC L, FABLET C, DORENLOR V, EONO F, EVENO E, JOLLY JP, MADEC F, KOBICH M
Comparaison de la PCR simple et de la PCR niché pour détecter M. hyopneumoniae chez le porc vivant [Comparison between PCR assay and nested PCR assay for the in vivo detection of M. hyopneumoniae in pigs].
Journées Recherche Porcine, 2007, Volume 39, 435-436
Five farrow-to-finish herds from Brittany (France) with various M. hyopneumoniae (Mhyo) herd status were included in this study. In each herd, 60 pigs were controlled from post-weaning to slaughter: nasal, tonsil and oropharingeal swabs collected at 4, 10, 16 and 22 weeks of age as well as lung samples collected at slaughter were assayed by PCR and nested PCR. In addition, a lung pneumonic lesion score was determined for each pig. A positive correlation was found between the severity of the lesions at slaughter and the percentage of pigs found positive by nested PCR. Moreover, a significant difference was obtained between the results of PCR and those of nested PCR, confirming that the latter has a higher sensitivity. However, the fact that the nested PCR technique requires an additional step compared to PCR increases the risks of contamination of the samples.
