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Bulletin No. 31 - Fall 2008
BacterologyHaemophilus parasuis
TURNI C, BLACKALL PJ
Comparison of sampling sites and detection methods for Haemophilus parasuis
Australian Veterinary Journal, 2007, Volume 85, Nº5, 177-184
As the isolation and identification of Haemophilus parasuis are often unsuccessful, the authors here tried to determine which tissues would be the best for the isolation of this bacterium. Two different trials were conducted, at a 5-month interval (serovar 4 challenge and serovar 12 challenge). In the serovar 4 challenge, gentamycin sulphate was administered to the pigs. Pigs were challenged with H. parasuis and necropsied on days 6 or 7 after challenge. Various tissues were sampled and tested either using conventional culture plates or a PCR assay. This study confirmed that PCR was not as efficient as conventional culture in the detection of H. parasuis, as PCR requires high numbers of H. parasuis to be able to detect these bacteria. Pleural fluid, peritoneal fibrin and fluid, lung and pericardial fluid proved to be the best tissues to test in the case of subacute infections (serovar 4). Conversely, the lungs, heart blood, affected joints and brain were the best sites for acute infections (serovar 12).
