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Bulletin No. 32 - Winter 2008
VirologyPMWS-PCV2-PCVD-PDNS
YU S, VINCENT A, OPRIESSNIG T, CARPENTER S, KITIKOON P, HALBUR PG, THACKER E
Quantification of PCV2 capsid transcript in peripheral blood mononuclear cells (PBMCs) in vitro
Veterinary Microbiology, 2007, Volume 123, 34-42
The objective of this study was to determine if an immune stimulation could trigger an increased PCV2 replication in PBMCs and thus play a role in the development of clinical disease. In this regard, the replication rate was evaluated in resting or concanavalin A (ConA) stimulated PBMCs. The levels of PCV2 DNA and capsid mRNA (Cap mRNA) were assessed using a real-time PCR and a real-time RT-PCR, respectively. The results showed that PBMCs did support the replication of PCV2, which increased over time. PCV2 replication was also significantly enhanced when PBMCs were stimulated with ConA. PCV2 replication was also tested in the presence of activated T lymphocytes and the results showed that the presence of these cells also had an impact on PCV2 replication.
