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Pig Journal Volume: 53
Publication date: May 2004

Proceedings Section

DEVELOPMENT OF A COMPETITIVE ELISA FOR THE DETECTION OF SERUM ANTIBODIES TO LEPTOSPIRA INTERROGANS SEROVAR BRATISLAVA
C. Frizzell, D.P. Mackie, J.M. Montgomery and W.A. Ellis

Abstract
Serological data indicates that Leptospira interrogans serovar Bratislava is the major pig maintained infection worldwide. Culture is extremely difficult and serology based on the microscopic agglutination test (MAT) requires live antigen and specialist laboratories. A competitive ELISA, based on a unique monoclonal antibody to serovar Bratislava has been developed for the detection of serum antibodies in pigs. Sera from vaccinated, experimentally infected and naturally infected pigs were used in evaluating the cELISA. The Bratislava cELISA is safe and easy to use and is suitable for screening large numbers of sera.

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