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Pig Journal Volume: 59
Publication date: July 2007

Proceedings Section

THE USE OF PLATE COUNT AGAR AND PETRIFILM™FOR THE ENUMERATION OF VIABLE ORGANISMS
G. Woodhead, A. Pritchett and M.A. Jones

Abstract
Two techniques for enumerating viable aerobic organisms in 41 samples of extended boar semen containing antibiotics were compared. High total viable counts were found in many samples by both techniques. There was no correlation between the simpler Petrifilm™ method and the spiral and spread plating techniques on plate count agar. In some cases, the Petrifilm™ grossly under-estimated the number of microbes present due to the effects of inhibitory substances produced by some bacteria.

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