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Pig Journal Volume: 60
Publication date: December 2007

Clinical Practice

AUJESZKY'S DISEASE: SYMPTOMS, EPIDEMIOLOGY AND NOVEL DIAGNOSTIC METHODS FOR ERADICATION
N.T. Kavanagh

Abstract
In this paper, the history of Aujeszky's disease (AD) is described, including vaccine development and eradication programmes. This was a background to a study in which the meat juice ELISA was used in comparison with serology to see if it would provide a low cost method of monitoring the AD status of pig herds in Ireland. The clinical signs of AD are reviewed and also the pathology differential diagnoses of AD, diagnostic tests, virus survival, epidemiology and serological monitoring are reviewed.

The main objective of the study was to compare the meat juice ELISA with standard serology, using the IDEXX gE antibody ELISA test kit. Meat juice at a concentration of 25% gave results similar to serum. Ten percent concentration resulted in a high percentage of false negatives. No false positives were given at 25% meat juice. It was concluded that a concentration of 20% meat juice combined with overnight incubation produced results which satisfied the objectives of the study. It was at least as sensitive as serum in an AD positive herd and gave no false positives in the two Aujeszky's disease free herds.

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