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Pig Journal Volume: 49
Publication date: June 2002

Refereed Section

A MODEL FOR THE EXPERIMENTAL INDUCTION OF GL?SSER'S DISEASE IN PIGLETS
by B. de Bleser, J.H. Morgan, S. Eassty and L. Gardey

Abstract
Two experimental infection studies were performed to establish a reproducible model for Gl?sser's Disease (GD) in five to six-week-old weaner gilts. In the first study, five isolates of Haemophilus parasuis were screened for their potential pathogenicity using intravenous (IV) injection of a challenge dose containing 109 colony-forming units (CFU). Only one strain induced acute clinical signs and pathology consistent with GD, with clinical onset within 15 hours. To obtain a less acute experimental disease with a longer incubation period, the second study used the virulent strain with lower doses by IV injection (108 and 107 CFU per piglet) and also used the intraperitoneal (IP) route of administration (using 109 and 108 CFU). Both infection protocols resulted in clinical GD, with seven out of eight piglets affected by IP challenge, and five out of eight affected by IV challenge. Results showed that not all field isolates of Haemophilus parasuis are virulent, whilst the route of infection and the challenge dose used seem to be important factors in determining the outcome of experimental infection. For production of an acute model of disease for efficacy testing of antimicrobials, a dose of approximately 108 cfu of virulent strain CRL 0037/001/00 by intraperitoneal injection is recommended.

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