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Pig Journal Volume: 46
Publication date: November 2000

Refereed Section

EVALUATION OF ELISA TEST FOR THE DIAGNOSIS OF PORCINE TRICHINELLOSIS
M. Ribicich, M. Miguez, A. Franco, N. Basso, R.H. Gamble, G. Santillan, V. Molina and E. Guarnera

Abstract
The purpose of this work was to validate the ELISA technique for the detection of porcine trichinellosis. Fourteen pigs were experimentally inoculated with doses of 0, 100, 500, 5000 or 50000 larvae of Trichinella spiralis. Inoculated animals were bled periodically to obtain serum. At the termination of the experiment (100 days post-inoculation), pigs were euthanized, skeletal muscle tissue was collected and the rapid artificial digestion method (DAR) used to determine parasite burden. ELISA values for animals in group 0 (uninoculated) remained under the positive cut-off during the entire experimental period (100 days). Inoculated animals developed antibody titers between days 21 and 56 post-inoculation. The ELISA test using Trichinella spiralis excretory/secretory antigen showed 100% sensitivity, and a 100% positive predictive value. Based on these results, it would be advisable to make use of the ELISA test as a tool for epidemiological monitoring in hog production establishments in Argentina and to use the rapid artificial digestion method for pigs in meat-packing plants.

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