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PMWS Research Archives
Published Feb 2012: J Virol Methods. 2012 Feb;179(2):390-5. Epub 2011 Dec 6.Development of a Loop-Mediated Isothermal Amplification Method for Rapid Detection of Porcine Boca-like Virus.
Li B, Ma JJ, Xiao SB, Zhang XH, Wen LB, Mao L, Ni YX, Guo RL, Zhou JM, Lv LX, He KW.
The porcine boca-like virus (Pbo-likeV) was recently discovered in Swedish pigs with post-weaning multisystemic wasting syndrome (PMWS). In this study, a loop-mediated isothermal amplification (LAMP) assay was developed for rapid, specific and sensitive...
Published Feb 2012: Journal of Virological Methods Volume 179, Issue 2, February 2012, Pages 311–315
Development of a Plexor Real-Time PCR Assay for the Detection of Porcine Circovirus Type 2
Michaela Vlasakova, Anna Jackova, Valeria Leskova, Stefan Vilcek
A novel, real-time PCR system for the detection of porcine circovirus type 2 (PCV2) was developed. The system employed Plexor technology and detected 108–101 copies per reaction of PCV2 DNA within a recombinant plasmid. The examination...
Published Feb 2012: Journal of Virological Methods Volume 179, Issue 2, February 2012, Pages 359–366
Baculovirus as a PRRSV and PCV2 Bivalent Vaccine Vector: Baculovirus Virions Displaying Simultaneously GP5 Glycoprotein of PRRSV and Capsid Protein of PCV2
Xin-Gang Xu, Zhi-Sheng Wang, Qi Zhang, Zhao-Cai Li, Li Ding, Wei Li, Hung-Yi Wu, Ching-Dong Chang, Long-Huw Leed, De-Wen Tong, Hung-Jen Liu
The GP5 glycoprotein of PRRSV is the main target for inducing neutralizing antibodies and protective immunity in the natural host. The capsid (Cap) protein is the major immunogenic protein and associated with the production of PCV2-specific neutralizing...
Published Jan 2012: J. Virol. January 2012
Complete Genome Sequence of a Novel Porcine Circovirus-Like Agent
LiBin Wen, KongWang He, ZhengYu Yu, AiHua Mao, YanXiu Ni, XueHan Zhang, RongLi Guo, Bin Li, XiaoMin Wang, JunMing Zhou and LiXin Lv
We report here the genome sequence of a porcine circovirus-like agent. The sequenced genome of this porcine circovirus-like agent is composed of a 648-nucleotide circular DNA that includes three predicted protein-coding genes, which means the agent should...
Published Jan 2012: Infection, Genetics and Evolution, In Press, Uncorrected Proof, Available online 24 January 2012
Detection of Natural Inter- and Intra-Genotype Recombination Events Revealed by Cap Gene Analysis and Decreasing Prevalence of PCV2 in Wild Boars
Dániel Cadar, Attila Cságola, Márta Lőrincz, Kata Tombácz, Marina Spînu, Tamás Tuboly
Porcine circovirus type 2 (PCV2), the causative agent of a number of PCVAD (porcine circovirus associated diseases), is ubiquitous in domestic pig and wild boar populations. In the present study, using recombination detection program, phylogenetic analysis...
Published Jan 2012: Virus Research, In Press, Accepted Manuscript, Available online 24 January 2012
Identification of an Emerging Recombinant Cluster in Porcine Circovirus Type 2
Lin Cai, Jianqiang Ni, Yingju Xia, Zhanchao Zi, Kun Ning, Peng Qiu, Xiaoxia Li, Baoyue Wang, Qi Liu, Dongmei Hu, Xiuling Yu, Zhi Zhou, Xinyan Zhai, Xue Han, Kegong Tian
Porcine circovirus type 2 (PCV2) is continuously evolving through point mutation and genome recombination. In the present study, genetic affiliations of 40 PCV2 strains were determined by amplification, sequencing and phylogenetic analyses during the...
Published Jan 2012: Virology, Volume 422, Issue 2, 20 January 2012, Pages 161-164
Low levels of Diversity Among Genomes of Porcine Circovirus Type 1 (PCV1) Points to Differential Adaptive Selection Between Porcine Circoviruses
Martà Cortey, Joaquim Segalés
Several features related with the evolutionary patterns among all the PCV1 genomes available at GenBank have been analyzed in the present work (diversity, number of genotypes, recombination, saturation, selection, evolutionary rate). The reported results...
Published Jan 2012: Transbound Emerg Dis. 2012 Jan 17. doi: 10.1111/j.1865-1682.2011.01288.x. [Epub ahead of print]
Porcine Circovirus Diseases: A review of PMWS.
Baekbo P, Kristensen CS, Larsen LE.
This article is a review on post-weaning multisystemic wasting syndrome (PMWS), the first described disease among the porcine circovirus diseases (PCVD). Post-weaning multisystemic wasting syndrome has, since its appearance in Canada in 1991, been seen...
Published Jan 2012: Veterinary Microbiology, In Press, Uncorrected Proof, Available online 17 January 2012
Oral Vaccination with Attenuated Salmonella enterica Serovar Typhimurium Expressing Cap protein of PCV2 and its Immunogenicity in Mouse and Swine Models
Xin-Gang Xu, Hong-Ni Zhao, Qi Zhang, Li Ding, Zhao-Cai Li, Wei Li, Hung-Yi Wu, Kuo Pin Chuang, De-Wen Tong, Hung-Jen Liu
Attenuated Salmonella enterica serovar Typhimurium (S. typhimurium) was selected as a transgenic vehicle for the development of oral vaccines against Porcine circovirus type 2 (PCV2). The Cap-encoding gene of PCV2 was amplified by PCR and...
Published Jan 2012: Vaccine. 2012 Jan 14. [Epub ahead of print]
Inactivated PCV2 One Shot Vaccine Applied in 3-week-old Piglets: Improvement of Production Parameters and Interaction with Maternally Derived Immunity.
Fraile L, Grau-Roma L, Sarasola P, Sinovas N, NofrarÃas M, López-Jimenez R, López-Soria S, Sibila M, Segalés J.
The present study describes the effects of a commercially available vaccine against Porcine circovirus type 2 (PCV2) on clinical, pathological and virological outcomes of 3-week-old piglets from two farms with a clinical history of postweaning multisystemic...
Published Jan 2012: Virus Research, In Press, Corrected Proof, Available online 14 January 2012
Naked PCV-2 Cloned Genomic DNA is Infectious by Mucosal (Intratracheal or Oro-Nasal) Inoculation
Béatrice Grasland, Lionel Bigault, Maëna Le Corvec, Anne-Cécile Hernandez-Nignol, Aurélie Oger, Daniel Dory, Nicolas Rose, Roland Cariolet, André Jestin
Porcine circovirus type 2 (PCV-2) is involved in several diseases named porcine circovirus-associated diseases and is transmitted by oro-faecal route. In this study we inoculated porcine-circovirus free piglets by mucosal routes (intratracheal or oro-nasal...
Published Jan 2012: Vet Rec. 2011 Nov 18. [Epub ahead of print]
Acute Phase Proteins as a tool for Differential Diagnosis of Wasting Diseases in Growing Pigs.
Quereda JJ, Gómez S, Seva J, Ramis G, Cerón JJ, Muñoz A, Pallarés FJ.
The concentrations of haptoglobin (Hp), C-reactive protein (CRP) and serum amyloid A (SAA) were measured in wasted pigs, first to evaluate their usefulness in the diagnosis of infectious, wasting diseases in pigs, and second, to evaluate whether their...
Published Jan 2012: Virus Research, Volume 163, Issue 1, January 2012, Pages 320-327
Tripping over Emerging Pathogens Around the World: A Phylogeographical Approach for Determining the Epidemiology of Porcine Circovirus-2 (PCV-2), Considering Global Trading
Pedro M.P. Vidigal, Claudio L. Mafra, Fernanda M.F. Silva, Juliana L.R. Fietto, Abelardo Silva Júnior, Márcia R. Almeida
Porcine circovirus-2 (PCV-2) is an emerging virus associated with a number of different syndromes in pigs known as Porcine Circovirus Associated Diseases (PCVAD). Since its identification and characterization in the early 1990s, PCV-2 has achieved a worldwide...
Published Dec 2011: Pig Journal Volume: 66
Unusual Manifestation of Porcine Circovirus-Associated Disease (PCVAD) among Vaccinated Grower Finisher Pigs With Isolation of PCV2 from Tissues
B.W. Strugnell, M.E.C.White, R.J. Higgins, G. Ibata, S. Grierson, M. Banks
Vaccines against Porcine Circovirus type 2 (PCV2) have recently become available in the UK and have changed the landscape of Porcine Circovirus Associated Disease (PCVAD), resulting in significant improvements in growing pig performance,mortality rates...
Published Dec 2011: Pig Journal Volume: 66
PMWS and PCV2 Associated Diseases: Historical Progress and a Reflection about Disease Emergence in Pigs
F. Madec
Post-weaning Multisystemic Wasting Syndrome (PMWS) was first described in 1996 in North America, and soon spread worldwide. The pig industry and scientific community witnessed the devastating consequences with dismay. Intensive research was carried out...
