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PMWS & PCVD

PMWS Research Archives

Published Jan 2013: Vaccine - Volume 31, Issue 3, 7 January 2013, Pages 487–494
A PCV2 Vaccine Based on Genotype 2b is more Effective than a 2a-based Vaccine to Protect against PCV2b or Combined PCV2a/2b Viremia in Pigs with Concurrent PCV2, PRRSV and PPV Infection
Tanja Opriessnig, Kevin O’Neill, Priscilla F. Gerber, Alessandra M.M.G. de Castro, Luis G. Gimenéz-Lirola, Nathan M. Beach, Lei Zhou, Xiang-Jin Meng, Chong Wang, Patrick G. Halbur
The predominant genotype of porcine circovirus (PCV) in the pig population today is PCV2b yet PCV2a-based commercial vaccines are considered effective in protecting against porcine circovirus associated disease. The objective of this study was to compare...

Published Jan 2013: Journal of Virological Methods - Volume 187, Issue 1, January 2013, Pages 15–19
Simultaneous Detection of Porcine Parvovirus And Porcine Circovirus Type 2 By Duplex Real-Time PCR And Amplicon Melting Curve Analysis using SYBR Green
Lan-lan Zheng, Ya-bin Wang, Ming-feng Li, Hong-ying Chen, Xian-po Guo, Jing-wei Geng, Zhen-ya Wang, Zhan-yong Wei, Bao-an Cui
The development of a SYBR Green-based duplex real-time PCR is described for simultaneous detection of porcine parvovirus (PPV) and porcine circovirus type 2 (PCV-2) genomes. Viral genomes were identified in the same sample by their distinctive melting...

Published Jan 2013: Preventive Veterinary Medicine - Volume 108, Issue 1, 1 January 2013, Pages 63–72
Investigation of the Association of Growth Rate in Grower-Finishing Pigs with the Quantification of Lawsonia intracellularis and Porcine Circovirus Type 2
Markku Johansen, MaiBritt Nielsen, Jan Dahl, Birgitta Svensmark, Poul Bækbo, Charlotte Sonne Kristensen, Charlotte Kristiane Hjulsager, Tim K. Jensen, Marie Ståhl, Lars E. Larsen, Øystein Angen
As a part of a prospective cohort study in four herds, a nested case control study was carried out. Five slow growing pigs (cases) and five fast growing pigs (controls) out of 60 pigs were selected for euthanasia and laboratory examination at the end...

Published Dec 2012: Veterinary Microbiology - Volume 161, Issues 1–2, 28 December 2012, Pages 229–234
Effect of Sow and Piglet Porcine Circovirus Type 2 (PCV2) Vaccination on Piglet Mortality, Viraemia, Antibody Titre and Production Parameters
Lorenzo Fraile, Marina Sibila, Miquel Nofrarías, Rosa López-Jimenez, Eva Huerta, Anna Llorens, Sergio López-Soria, Diego Pérez, Joaquim Segalés
The present study describes the effects of sow and/or piglet porcine circovirus type 2 (PCV2) vaccination on viraemia, antibody response and production parameters (average daily weight gain [ADWG] and mortality) of piglets from a PCV2 subclinically infected...

Published Dec 2012: Veterinary Microbiology - Available online 22 December 2012 -In Press, Accepted Manuscript
Emergence of a Novel Mutant PCV2b Variant Associated with Clinical PCVAD in Two Vaccinated Pig Farms in the U.S. Concurrently Infected with PPV2
Tanja Opriessnig, Chao-Ting Xiao, Priscilla F. Gerber, Patrick G. Halbur
Porcine circovirus (PCV) type 2b (PCV2b) emerged in North America in 2005-2006. During May of 2012, PCVAD occurred in 10-to-18-week-old pigs in two farms within a production system that routinely vaccinated against PCV2. Both farms received replacement...

Published Dec 2012: Research in Veterinary Science - Available online 21 December 2012 In Press, Corrected Proof
Prevalence and Genetic Variation of Porcine Circovirus Type 2 in Taiwan from 2001 to 2011
Chun Wang, Victor Fei Pang, Fan Lee, Tien-Shine Huang, Shu-Hwae Lee, Yu-Ju Lin, Yeou-Liang Lin, Shiow-Suey Lai, Chian-Ren Jeng
Porcine circovirus type 2 (PCV2) is the major causative agent of postweaning multisystemic wasting syndrome (PMWS) in Taiwanese pig farms. We analyzed the complete genomes of 571 Taiwanese PCV2 isolates in Taiwan from 2001 to 2011 and divided the isolates...

Published Dec 2012: Veterinary Microbiology - Available online 20 December 2012 - In Press, Corrected Proof
Construction and Immunogenicity of Recombinant Porcine Circovirus-like Particles Displaying Somatostatin
Wenliang Li, Xianwei Wang, Juan Bai, Tao Ma, Zhijun Li, Yufeng Li, Ping Jiang
In order to obtain a virus-like particles (VLPs) vaccine both for porcine circovirus type 2 (PCV2) prevention and growth-promotion, somatostatin (SS) gene was fused to the 3'-terminal of ORF2 gene of PCV2 with PCR, and a recombinant baculovirus (rAc-Cap-SS)...

Published Dec 2012: Vaccine - Volume 31, Issue 1, 17 December 2012, Pages 141–148
Antibody Repertoire Development in Fetal and Neonatal Piglets. XV. Porcine Circovirus Type 2 Infection Differentially Affects Serum IgG Levels and Antibodies to ORF2 in Piglets Free From Other Environmental Factors
Xiuzhu Sun, N. Wertz, K.M. Lager, J.E. Butler
Porcine circovirus type 2 (PCV2) is an important pathogen in the porcine respiratory disease complex (PRDC) and its persistence may be due to dysregulation of systemic immunity. We examined this contention using isolator piglets. We present data on Ig...

Published Dec 2012: Veterinary Microbiology, In Press, Corrected Proof, Available online 13 December 2012
Innate Pro-Inflammatory and Adaptive Immune Cytokines in PBMC of Vaccinated and Unvaccinated Pigs Naturally Exposed to Porcine Circovirus Type 2 (PCV2) Infection vary with the Occurrence of the Disease and the Viral Burden
Paolo Borghetti, Marina Morganti, Roberta Saleri, Luca Ferrari, Elena De Angelis, Valeria Cavalli, Antonio Cacchioli, Attilio Corradi, Paolo Martelli
Pro-inflammatory (IL-8, TNF-α, IL-1ß) and immune (IFN-γ, IL-10) cytokines were evaluated in PCV2-vaccinated and unvaccinated pigs exposed to natural PCV2 infection retrospectively selected according to the time of the onset of viremia...

Published Dec 2012: Research in Veterinary Science Volume 93, Issue 3, December 2012, Pages 1520–1524
Culturing and Molecular Methods to Assess the Infectivity of Porcine Circovirus from Treated Effluent of Swine Manure
A. Viancelli, L.A.T. Garcia, M. Schiochet, A. Kunz, R. Steinmetz, J.R. Ciacci-Zanella, P.A. Esteves, C.R.M. Barardi
Samples were collected at the effluent of two swine manure treatment systems and were analyzed by qPCR to determine the presence and amounts of porcine circovirus (PCV2) genetic material. ST cells were inoculated with the positive samples to evaluate...

Published Dec 2012: Research in Veterinary Science, Volume 93, Issue 3, December 2012, Pages 1231-1240
Infectious Risk Factors for Individual Postweaning Multisystemic Wasting Syndrome (PMWS) Development in Pigs from Affected Farms in Spain and Denmark
Llorenç Grau-Roma, Anders Stockmarr, Charlotte S. Kristensen, Claes Enøe, Sergio López-Soria, Miquel Nofrarías, Vivi Bille-Hansen, Charlotte K. Hjulsager, Marina Sibila, Sven E. Jorsal, Lorenzo Fraile, Poul Baekbo, Hakan Vigre, Joaquim Segalés, Lars
Two prospective longitudinal studies in 13 postweaning multisystemic wasting syndrome (PMWS)-affected farms from Spain (n = 3) and Denmark (n = 10) were performed. Blood samples from pigs were longitudinally collected from 1st week until the occurrence...

Published Dec 2012: Research in Veterinary Science, Volume 93, Issue 3, December 2012, Pages 1525-1530
PCV2 Induces Apoptosis and Modulates Calcium Homeostasis in Piglet Lymphocytes In Vitro
Yingjun Lv, Lei Dai, Huili Han, Shuxia Zhang
This study investigated the process of PCV2-induced apoptosis and the effect of PCV2 inoculation on calcium homeostasis in piglet lymphocytes in vitro. PCV2-inoculated lymphocytes exhibited chromatin condensation, chromatin segregation, the appearance...

Published Nov 2012: Veterinary Immunology and Immunopathology, Volume 150, Issues 1–2, 15 November 2012, Pages 128-132
Evaluation of Cell-Mediated Immune Responses against Porcine Circovirus Type 2 (PCV2) Cap and Rep Proteins after Vaccination with a Commercial PCV2 Sub-Unit Vaccine
Maria Fort, Marina Sibila, Miquel Nofrarías, Eva Pérez-Martín, Alex Olvera, Enric Mateu, Joaquim Segalés
This study investigated the development of cellular immunity to Porcine circovirus type 2 (PCV2) Cap and Rep proteins in pigs vaccinated with a commercial PCV2 genotype a (PCV2a) based sub-unit vaccine, before and after a heterologous challenge with a...

Published Nov 2012: Research in Veterinary Science, In Press, Corrected Proof, Available online 8 November 2012
Verification of Natural Infection of Peridomestic Rodents by PCV2 on Commercial Swine Farms
Albanno Leonard Braz Campos Pinheiro, Luiz Henrique Silva Bulos, Thiago Souza Onofre, Michelle de Paula Gabardo, Otávio Valério de Carvalho, Mariana Costa Fausto, Roberto Maurício Carvalho Guedes, Márcia Rogéria de Almeida, Abelardo Silva Júnior
The porcine circovirus-2 (PCV2) is the main agent responsible for porcine circovirus associated diseases (PCVAD). Few studies have been done regarding PCV2 infection in other species. The purpose of this study was to investigate the occurrence of PCV2...

Published Oct 2012: Research in Veterinary Science Volume 93, Issue 2, October 2012, Pages 1042–1045
Detection and Molecular Characterization of Porcine Circovirus Type 2 (PCV2) from Piglets with Exudative Epidermitis in Uruguay
Ramos N, Mirazo S, Castro G, Arbiza J.
Porcine circovirus type 2 (PCV2) is an economically important emerging pathogen associated with distinct syndromes and diseases in swine, collectively known as porcine circovirus associated diseases (PCVAD). The main purpose of this study was to investigate...

 
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