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Vaccination
Management
Disease Information
A PMWS update (Jake Waddilove)
ABOUT PMWS & PDNS National Pork Board PMWS Fact Sheet About PDNS (Jake Waddilive) CEI Emerging Disease Notices: PMWS / PDNS Conference and meetings archive
Case Histories
Yorkshire Farm, UK - Mike Muirhead - Final Update, June 2002
Mike Muirhead's case history of a Yorkshire farm with PMWS and PDNS. This paper charts the course and effects of the disease on a single herd as well as highlighting the economic impact. Photographs
Clinical signs
Photos of the clinical signs that are seen generally in pigs with PMWS and PDNS. Includes skin lesions, enlarged lymph glands, wasting and dead pigs. Photos of the signs that are seen in post-mortem samples of pigs with PMWS and PDNS. Includes interstitial pneumonia, secondary bacterial infection, enlarged lymph nodes, oedema and intra cytoplasmic inclusions More Photos of the signs that are seen in post-mortem samples of pigs with PMWS and PDNS.
PMWS Research ArchivesPublished Friday, May 02, 2008: Journal of Virological Methods Volume 149, Issue 2, May 2008, Pages 264-268Rapid detection of porcine circovirus type 2 by loop-mediated isothermal amplification Hao-tai Chen, Jie Zhang, De-hui Sun, Yue-feng Chu, Xue-peng Cai, Xiang-tao Liu, Xue-nong Luo, Qing Liu and Yong-sheng Liu A method of loop-mediated isothermal amplification (LAMP) was employed to develop a rapid and simple detection system for porcine circovirus type 2 (PCV2). The amplification could be finished in 60 min under isothermal condition at 64 ēC by employing a set of four primers targeting the cap gene of PCV2. The LAMP assay showed higher sensitivity than the conventional PCR, with a detection limit of five copies per tube of purified PCV2 genomic DNA. No cross-reactivity was observed from the samples of other related viruses including porcine circovirus type 1 (PCV1), porcine parvovirus (PPV), porcine pseudorabies virus (PRV) and porcine reproductive and respiratory syndrome virus (PRRSV). The detection rate of PCV2 LAMP for 86 clinical samples was 96.5% and appeared greater than that of the PCR method. The LAMP assay reported can provide a rapid yet simple test of PCV2 suitable for laboratory diagnosis and pen-side detection due to ease of operation and the requirement of only a regular water bath or heat block for the reaction. To continue reading this article please click here Have you published information? To add please email the details |















