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Vaccination
Management
Disease Information
A PMWS update (Jake Waddilove)
ABOUT PMWS & PDNS National Pork Board PMWS Fact Sheet About PDNS (Jake Waddilive) CEI Emerging Disease Notices: PMWS / PDNS Conference and meetings archive
Case Histories
Yorkshire Farm, UK - Mike Muirhead - Final Update, June 2002
Mike Muirhead's case history of a Yorkshire farm with PMWS and PDNS. This paper charts the course and effects of the disease on a single herd as well as highlighting the economic impact. Photographs
Clinical signs
Photos of the clinical signs that are seen generally in pigs with PMWS and PDNS. Includes skin lesions, enlarged lymph glands, wasting and dead pigs. Photos of the signs that are seen in post-mortem samples of pigs with PMWS and PDNS. Includes interstitial pneumonia, secondary bacterial infection, enlarged lymph nodes, oedema and intra cytoplasmic inclusions More Photos of the signs that are seen in post-mortem samples of pigs with PMWS and PDNS.
PMWS Research ArchivesPublished Tuesday, September 01, 2009: Journal of Virological Methods, Volume 160, Issues 1-2, September 2009, Pages 210-214Multiplex PCR and Multiplex RT-PCR for Inclusive Detection of Major Swine DNA and RNA Viruses in Pigs with Multiple Infections Hirohito Ogawa, Osamu Taira, Takuya Hirai, Hiromi Takeuchi, Aki Nagao, Yoshiki Ishikawa, Kotaro Tuchiya, Tetsuo Nunoya, Susumu Ueda Multiplex PCR and multiplex RT-PCR were developed to identify nine viruses in pigs with multiple infections. These viruses are: porcine circovirus type 2 (PCV2), suid herpesvirus 1, porcine parvovirus (PPV), porcine reproductive and respiratory syndrome virus (PRRSV), Japanese encephalitis virus, porcine rotavirus A (PoRV-A), porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), and Getah virus. These methods were shown to be high specificity and sensitivity. In the clinical application, a total of 75 field samples were examined by our methods and previously reported methods for PCV2, PRRSV, TGEV, and PEDV. As a result, the detection rates of our multiplex PCR and multiplex RT-PCR were higher than those of the previously reported methods. Furthermore, it was confirmed that 24 PCV2 positive samples were co-infected with other viruses, 11 with PRRSV, 10 with PPV, 2 with PoRV-A, and 1 with TGEV by a combination of multiplex PCR and multiplex RT-PCR. PPV and PoRV-A were newly detected by multiplex PCR and multiplex RT-PCR. These results suggest that the combination of our multiplex PCR and multiplex RT-PCR is useful for rapid and accurate identification of nine major pathogenic viruses in pigs with multiple infections. To continue reading this article please click here Have you published information? To add please email the details |








