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Vaccination
Management
Disease Information
A PMWS update (Jake Waddilove)
ABOUT PMWS & PDNS
National Pork Board PMWS Fact Sheet
About PDNS (Jake Waddilive)
CEI Emerging Disease Notices: PMWS / PDNS
Conference and meetings archive
Case Histories
Yorkshire Farm, UK - Mike Muirhead - Final Update, June 2002
Mike Muirhead's case history of a Yorkshire farm with PMWS and PDNS.
East Anglia Farm, UK - Philip Richardson
This paper charts the course and effects of the disease on a single herd as well as highlighting the economic impact.
Photographs
Clinical signs
Photos of the clinical signs that are seen generally in pigs with PMWS and PDNS. Includes skin lesions, enlarged lymph glands, wasting and dead pigs.
Post mortem (1)
Photos of the signs that are seen in post-mortem samples of pigs with PMWS and PDNS. Includes interstitial pneumonia, secondary bacterial infection, enlarged lymph nodes, oedema and intra cytoplasmic inclusions
Post mortem (2)
More Photos of the signs that are seen in post-mortem samples of pigs with PMWS and PDNS.


PMWS Research Archives

Published Thursday, July 01, 2010: Analyst. 2010 Jul;135(7):1680-5. Epub 2010 May 27.
Ultrasensitive Detection of Porcine Circovirus Type 2 Using Gold(III) Enhanced Chemiluminescence Immunoassay.
Zhang H, Li W, Sheng Z, Han H, He Q.
Porcine circovirus type 2 (PCV2) is associated with many diseases especially postweaning multisystemic wasting syndrome (PMWS), which has brought huge economic loss to the swine industry worldwide. Viral detection will pave the way for this disease prevention. Herein, we developed a facile, rapid and ultrasensitive method for detection of PCV2 based on gold(III) enhanced chemiluminescence immunoassay (CLIA). The gold(III), dissolved from the gold nanoparticle-monoclonal antibody conjugate, served as an analyte for the indirect measurement of PCV2. Under the optimal conditions, the detection limit for the detection of PCV2 was 1.71 x 10(3) copies mL(-1). Moreover, the CLIA signal can be further enhanced by using hydroxylamine-amplified gold nanoparticles, and the limit of detection was as low as 2.67 x 10(2) copies mL(-1). Compared with conventional polymerase chain reaction (PCR), the proposed method has good sensitivity and reliability in analysis of 36 serum samples, and showed great potential in virus assays.

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