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Vaccination
Management
Disease Information
A PMWS update (Jake Waddilove)
ABOUT PMWS & PDNS
National Pork Board PMWS Fact Sheet
About PDNS (Jake Waddilive)
CEI Emerging Disease Notices: PMWS / PDNS
Conference and meetings archive
Case Histories
Yorkshire Farm, UK - Mike Muirhead - Final Update, June 2002
Mike Muirhead's case history of a Yorkshire farm with PMWS and PDNS.
East Anglia Farm, UK - Philip Richardson
This paper charts the course and effects of the disease on a single herd as well as highlighting the economic impact.
Photographs
Clinical signs
Photos of the clinical signs that are seen generally in pigs with PMWS and PDNS. Includes skin lesions, enlarged lymph glands, wasting and dead pigs.
Post mortem (1)
Photos of the signs that are seen in post-mortem samples of pigs with PMWS and PDNS. Includes interstitial pneumonia, secondary bacterial infection, enlarged lymph nodes, oedema and intra cytoplasmic inclusions
Post mortem (2)
More Photos of the signs that are seen in post-mortem samples of pigs with PMWS and PDNS.


PMWS Research Archives

Published Friday, June 10, 2011: Proteomics
Quantitative Proteomics by 2-DE, 18O/16O Labeling and Linear ION Trap Mass Spectrometry Analysis of Lymph Nodes from Piglets Inoculated by Porcine Circovirus Type 2 (PCV2)
María Ramírez-Boo1, Estefania Núnez, Inmaculada Jorge, Pedro Navarro, Lana T. Fernandes, Joaquim Segalés, Juan J. Garrido1, Jesús Vázquez, Ángela Moreno1
Porcine circovirus type 2 (PCV-2) has been identified as the essential causal agent of postweaning multisystemic wasting syndrome (PMWS). However, little is known regarding the mechanism(s) underlying the pathogenesis of PCV-2-induced disease and the interaction of the virus with the host immune system. Here, we present a proteomics study on inguinal lymph nodes of piglets inoculated with PCV2, in order to better understand the pathogenesis of PMWS and the pathways might be affected after infection. We used two proteomics strategies, 2-DE and 1-D electrophoresis followed by 16O/18O peptide labeling and peptide identification and quantification by mass spectrometry. More than 100 proteins were found to be differentially regulated and the results obtained by the two strategies were fairly concordant but also complementary, the 18O labeling approach being a more robust alternative. Analysis of these proteins by systems biology tools revealed the implication of acute phase response and NrF2-mediated oxidative stress, suggesting a putative role for these pathways in the pig immune response. Besides, CD81 was found to be up-regulated, suggesting a possible role in the internalization of the virus. The use of proteomics technologies together with biology analysis systems opens up the way to gain more exhaustive and systematic knowledge of virus-pathogen interactions.

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