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PMWS Research Archives
Published Apr 2009: Veterinary Microbiology - Available online 10 April 2009Development and Evaluation of an Indirect in situ Polymerase Chain Reaction for the Detection of Porcine Circovirus Type 2 in Formalin-Fixed and Paraffin-Embedded Tissue Specimens
Chun-Ming Lin, Chian-Ren Jeng, Shih-Hsuan Hsiao, Chih-Cheng Chang, Chen-Hsuan Liu, Yi-Chieh Tsai, Mi-Yuan Chia and Victor Fei Pang
Taking advantage of the high sensitivity of polymerase chain reaction (PCR) and the cell-localizing ability of in situ hybridization (ISH), an indirect in situ PCR (ISPCR) method was developed for detecting the distribution of porcine circovirus...
Published Mar 2009: J Vet Med Sci. 2009 Mar;71(3):349-53
Genetic Diversity of Porcine Circovirus Type 2 in Korean Pigs with Postweaning Multisystemic Wasting Syndrome During 2005-2007.
Kim HH, Park SI, Hyun BH, Park SJ, Jeong YJ, Shin DJ, Chun YH, Hosmillo M, Lee BJ, Kang MI, Cho KO.
The comparison of nucleotide and deduced amino acid sequence was conducted with 9 porcine circovirus type 2 (PCV2) strains isolated from PCV2-infected pigs with postweaning multisystemic wasting syndrome (PMWS) and 50 tissue samples obtained from PCV2-infected...
Published Mar 2009: Vet Pathol. 2009 Jul;46(4):729-35. Epub Mar 9 2009 .
Ultrastructural findings in Lymph Nodes from Pigs Suffering from Naturally Occurring Postweaning Multisystemic Wasting Syndrome.
Rodriguez-Cariño C, Segalés J.
The aims of this study were to evaluate ultrastructural lesions in lymph nodes from postweaning multisystemic wasting syndrome (PMWS)-affected pigs and to correlate these alterations with detection of viral-like particles (VLPs). Samples of lymph nodes...
Published Mar 2009: Acta Vet Scand. 2009; 51(1): 13. Published online March 27 2009
The Index Herd with PMWS in Sweden: Presence of Serum Amyloid A, Circovirus 2 Viral Load and Antibody Levels in Healthy and PMWS-Affected Pigs
Per Wallgren, Inger Marit Brunborg, Gunilla Blomqvist, Gunnar Bergström, Frida Wikström, Gordon Allan, Caroline Fossum, and Christine Monceyron Jonassen
Background Postweaning Multisystemic Wasting Syndrome (PMWS) is an emerging disease in pigs of multifactorial origin, but associated to porcine circovirus type 2 (PCV2) infection. PMWS was first diagnosed in Sweden at a progeny test station that...
Published Mar 2009: J. Gen. Virol., Mar 2009; 90: 648 - 653
Swine torque teno virus detection in pig commercial vaccines, enzymes for laboratory use and human drugs containing components of porcine origin
Tuija Kekarainen, Laura Martínez-Guinó, and Joaquim Segalés
Torque teno viruses (TTVs) are vertebrate infecting, single-stranded circular DNA viruses. Two genetically distinct TTV genogroups (TTV1 and TTV2) infect swine worldwide with high prevalence. Currently, swine TTVs are considered non-pathogenic, although...
Published Mar 2009: Preventive Veterinary Medicine, Volume 88, Issue 3, 1 March 2009, Pages 213-219
The relationship between porcine circovirus 2 antigen score and antibody titre and histology of lymph nodes in 375 euthanased sick and healthy pigs from 113 British pig farms with and without postweaning multisystemic wasting syndrome.
M.J. Turner, G.F. Medley, K.A. Woodbine, J.A. Slevin, L.E. Green
Data from a cross-sectional study of 113 British pig herds carried out in 2004 were used to investigate the associations between postweaning multisystemic wasting (PMWS) in pigs and herds and porcine circovirus 2 (PCV2) antigen score and antibody titre,...
Published Mar 2009: Veterinary Microbiology, 134 (3), p.199-207, Mar 2009
Retrospective study on swine Torque teno virus genogroups 1 and 2 infection from 1985 to 2005 in Spain
Segales J, Martinez-Guino L, Cortey M, Navarro N, Huerta E, Sibila M., Pujols J, Kekarainen T
A retrospective study to detect evidence of swine Torque teno virus (TTV) genogroups 1 and 2 infection in sera of pigs from the Spanish livestock between years 1985 and 2005 was carried out by means of PCR. Also, the molecular evolution of TTV genogroups...
Published Feb 2009: Veterinary Microbiology February 2009
Retrospective Study on the Occurrence of Porcine Circovirus 2 Infection and Associated Entities in Northern Germany
Bjoern Jacobsen, Lars Krueger, Frank Seeliger, Michael Bruegmann, Joaquim Segalés and Wolfgang Baumgaertner
Porcine circovirus type 2 (PCV 2) represents a widespread, globally occurring pathogen with an increasing number of associated entities. To further elucidate the origin, spread and pathogenesis of PCV2 and associated changes archived material of pigs...
Published Feb 2009: Veterinary Microbiology February 2009.
In Utero Transmission of Porcine Torque Teno Viruses
Tanja Pozzuto, Bettina Mueller, Brian Meehan, Susan S. Ringler, Kathleen A. McIntosh, John A. Ellis, Annette Mankertz and Steven Krakowk
Sera and selected tissue homogenates collected from gnotobiotic swine never exposed to the environment or other swine tissues were tested for the presence of porcine torque teno virus (TTV) DNAs by nested and non-nested polymerase chain reactions (PCR)...
Published Feb 2009: Research in Veterinary Science Volume 86, Issue 1, February 2009, Pages 108-110
Evidence of shedding of porcine circovirus type 2 in milk from experimentally infected sows
Y. Ha, K.K. Ahn, B. Kim, K.-D. Cho, B.H. Lee, Y.-S. Oh, S.-H. Kim and C. Chae
Detection of porcine circovirus type 2 (PCV2) was to evaluate the milk from experimentally infected sows using polymerase chain reaction (PCR) and virus isolation. Six pregnant sows were inoculated intranasally with PCV2 at 93 days of gestation, and milk...
Published Jan 2009: Res Vet Sci. 2009 Jan 29.
Detection of porcine Circovirus type 2 (PCV2) variants PCV2-1 and PCV2-2 in Brazilian pig population.
Ciacci-Zanella JR, Simon NL, Pinto LS, Viancelli A, Fernandes LT, Hayashi M, Dellagostin OA, Esteves PA.
In the present study whole genome of six Brazilian isolates of PCV2 were sequenced, analyzed and compared with 35 other sequences (24 from other countries and 17 from Brazil). The phylogenetic analysis showed that mostly Brazilian variants of PCV2 were...
Published Jan 2009: Vet Record 2009 Jan 10;164(2):48-51.
Effects of a Modified live CSFV Vaccine on the Development of PMWS in Pigs Infected Experimentally with PCV-2.
Ha Y, Lee EM, Lee YH, Kim CH, Kim D, Chae S, Ahn KK, Kim B, Chae C.
The objective of this study was to determine the effect of vaccination against classical swine fever virus (CSFV) on the development of postweaning multisystemic wasting syndrome (PMWS) in conventional pigs infected experimentally with porcine circovirus...
Published Jan 2009: Canadian Journal Vet Res. 2009 January; 73(1): 21–24.
Retrospective Serological Survey of Porcine Circovirus-2 Infection in Mexico
Humberto Ramírez-Mendoza, Héctor Castillo-Juárez, Jesús Hernández, Pablo Correa, and Joaquim Segalés
Postweaning multisystemic wasting syndrome (PMWS) is considered a multifactorial emerging disease of which Porcine circovirus-2 (PCV-2) is the necessary infectious cause. However, retrospective studies have shown that PMWS is not a new disease and that...
Published Jan 2009: Veterinary Microbiology
Torque Teno virus (TTV) infection in sows and suckling piglets
M. Sibila, L. Martínez-Guinó, E. Huerta, M. Mora, L. Grau-Roma, T. Kekarainen and J. Segalés
Torque Teno virus (TTV) is a single stranded DNA virus that has been detected in serum of primate and non-primate species including swine. Little information on swine TTV infection and transmission dynamics is nowadays available. The goal of this study...
Published Jan 2009: Journal of Comparative Pathology
Detection of Porcine Circovirus 2 in Mammary and Other Tissues from Experimentally Infected Sows
J.-S. Park, Y. Ha, B. Kwon, K.D. Cho, B.H. Lee and C. Chae
The aim of this study was to determine whether porcine circovirus 2 (PCV2) may infect the mammary gland of sows and be shed in the milk. Six pregnant sows were inoculated intranasally with PCV2 three weeks before their expected farrowing date and two...
