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PMWS Research Archives
Published Jul 2008: Vet Microbiol. 2008 Jul 4.Inter-laboratory and inter-assay comparison on two real-time PCR techniques for quantification of PCV2 nucleic acid extracted from field samples.
Hjulsager CK, Grau-Roma L, Sibila M, Enøe C, Larsen L, Segalés J.
Several real-time PCR assays for quantification of PCV2 DNA (qPCR) have been described in the literature, and different in-house assays are being used by laboratories around the world. A general threshold of 10(7) copies of PCV2 per millilitre serum for...
Published Jul 2008: Vet Microbiol. 2008 Jul 7
A meta-analysis on experimental infections with porcine circovirus type 2 (PCV2).
Tomás A, Fernandes LT, Valero O, Segalés J.
A meta-analysis was performed with the aim to identify factors with a relevant influence on the expression of clinical postweaning multisystemic wasting syndrome (PMWS) under experimental conditions. Data from 44 studies were included in the analysis....
Published Jun 2008: The Veterinary Record, Jun 2008; 162: 867 - 868.
PCV-2 Genotype Definition and Nomenclature
J. Segalés, A. Olvera, L. Grau-Roma, C. Charreyre, H. Nauwynck, L. Larsen, K. Dupont, K. McCullough, J. Ellis, S. Krakowka, A. Mankertz, M. Fredholm, C. Fossum, S. Timmusk, N. Stockhofe-Zurwieden, V. Beattie, D. Armstrong, B. Grassland, P. Bae
No abstract is available for this article. See 'Journal...
Published Jun 2008: Veterinary Microbiology Volume 129, Issues 3-4, 22 June 2008, Pages 367-377
Changes in peripheral blood leukocyte subpopulations in piglets co-infected experimentally with porcine reproductive and respiratory syndrome virus and porcine circovirus type 2
Kaichuang Shi, Huanrong Li, Xin Guoa, Xinna Ge, Hong Jia, Shijun Zheng and Hanchun Yang
Porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) are pathogens, which can significantly affect the swine industry worldwide. Field surveys suggest that simultaneous PRRSV and PCV2 infection is common in...
Published Jun 2008: Vet Microbiol. 2008 Jun 20
Development and validation of a SYBR green real-time PCR for the quantification of porcine circovirus type 2 in serum, buffy coat, feces, and multiple tissues
McIntosh KA, Tumber A, Harding JC, Krakowka S, Ellis JA, Hill JE.
The emergence of multiple genotypes of PCV2, as demonstrated by phylogenetic analysis of whole genome or capsid sequences, makes it necessary to have quantitative diagnostic assays that perform equally well on all strains. The objectives of this study...
Published Jun 2008: Res Vet Sci. 2008 Jun;84(3):497-501. Epub 2007 Aug 14.
Genetic characterization of porcine circovirus type 2 in Republic of Korea.
Choi KS, Chae JS.
Porcine circovirus type 2 (PCV2) is a major causative agent of postweaning multisystemic wasting syndrome (PMWS). Sequence and phylogenetic analyses based on the ORF2 capsid protein gene fragment showed that field isolate in Republic of Korea (ROK), PCV2...
Published Jun 2008: Journal of Virological Methods Volume 150, Issues 1-2, June 2008, Pages 21-26
Construction and immunogenicity of recombinant pseudotype baculovirus expressing the capsid protein of porcine circovirus type 2 in mice
Huiying Fan, Yongfei Pan, Liurong Fang, Dang Wang, Shengping Wang, Yunbo Jiang, Huanchun Chen and Shaobo Xiao
Baculovirus has emerged recently as a novel and attractive gene delivery vehicle for mammalian cells. Porcine circovirus type 2 (PCV2) is known to be associated with post-weaning multisystemic wasting syndrome (PMWS), an emerging swine disease which results...
Published Jun 2008: Acta Vet Hung. 2008 Jun;56(2):207-13.
Gastric zygomycosis in a pig affected with postweaning multisystemic wasting syndrome--case report.
Szeredi L, Szentirmai C.
A postweaning pig died in spite of antibiotic therapy showing wasting in a small herd. Postweaning multisystemic wasting syndrome (PMWS) was diagnosed on the basis of gross pathological and histological lesions and the presence of moderate amounts of...
Published May 2008: Veterinary Microbiology Volume 129, Issues 1-2, 25 May 2008, Pages 97-107
Examination for a viral co-factor in postweaning multisystemic wasting syndrome (PMWS)
Louise Lohse, Anette Bøtner, Anne-Sofie Ladekjær Hansen, Tina Frederiksen, Kitt Dupont, Charlotte S. Christensen, Poul Bækbo and Jens Nielsen
In order to test the hypothesis that a putative co-factor for the development of postweaning multisystemic wasting syndrome (PMWS) in pigs could be of viral origin, we performed extensive virological examinations on organ material from pigs diagnosed...
Published May 2008: Journal of Virological Methods Volume 149, Issue 2, May 2008, Pages 264-268
Rapid detection of porcine circovirus type 2 by loop-mediated isothermal amplification
Hao-tai Chen, Jie Zhang, De-hui Sun, Yue-feng Chu, Xue-peng Cai, Xiang-tao Liu, Xue-nong Luo, Qing Liu and Yong-sheng Liu
A method of loop-mediated isothermal amplification (LAMP) was employed to develop a rapid and simple detection system for porcine circovirus type 2 (PCV2). The amplification could be finished in 60 min under isothermal condition at 64 ºC by employing...
Published May 2008: The Veterinary Journal Volume 176, Issue 2, May 2008, Pages 158-169
A review of viral diseases of the European wild boar: Effects of population dynamics and reservoir rôle
Francisco Ruiz-Fons, Joaquim Segalés and Christian Gortázar
There has been a worldwide increase in the number and geographical spread of wild boar populations in recent decades leading to an increase in both the circulation of disease agents and greater contact with domestic animals and humans. Diseases affect...
Published May 2008: J Vet Med Sci. 2008 May;70(5):497-500.
A piglet with concurrent polioencephalomyelitis due to porcine teschovirus and postweaning multisystemic wasting syndrome.
Takahashi M, Seimiya YM, Seki Y, Yamada M.
A piglet developed respiratory distress followed by difficulty in standing and unsteady gait. The lesions were characterized by polioencephalomyelitis with the predominant distribution in the brain stem, as well as lymphocyte depletion and histiocyte...
Published May 2008: Vaccine. 2008 May 5. (Epub ahead of print)
Reduction of PMWS-associated clinical signs and co-infections by vaccination against PCV2.
Kixmöller M, Ritzmann M, Eddicks M, Saalmüller A, Elbers K, Fachinger V.
The effects of a single-dose recombinant Porcine circovirus type 2 (PCV2) open reading frame 2 (ORF2) subunit vaccine were studied in a post-weaning multisystemic wasting syndrome (PMWS)-affected pig herd. A total of 1519 3-week-old piglets were allocated...
Published May 2008: J Vet Diagn Invest. 2008 May;20(3):283-8
Evaluation of a nested polymerase chain reaction assay to differentiate between two genotypes of Porcine circovirus-2.
Lyoo KS, Kim HB, Joo HS.
Porcine circovirus-2 (PCV-2) is associated with several diseases in pigs, including postweaning multisystemic wasting syndrome (PMWS). A new genotype of PCV-2 was isolated from swine farms with and without clinical PMWS in North America. The new genotype...
Published May 2008: Journal of Virological Methods Volume 149, Issue 2, May 2008, Pages 217-225
Development and validation of a duplex real-time PCR assay for the simultaneous detection and quantification of porcine circovirus type 2 and an internal control on porcine semen samples
N. Pal, Y.W. Huang, D.M. Madson, C. Kuster, X.J. Meng, P.G. Halbur and T. Opriessnig
A duplex real-time quantitative PCR (qPCR) method for the simultaneous detection of porcine circovirus type 2 (PCV2) and an exogenous internal positive control (IPC) in porcine semen samples was developed. The IPC was included to monitor DNA extraction...
