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PMWS Research Archives
Published Jan 2009: Canadian Journal Vet Res. 2009 January; 73(1): 7–14.Ring Tests to Evaluate the Performance of Porcine Circovirus-2 (PCV-2) Polymerase Chain Reaction (PCR) Assays used in North American Diagnostic Laboratories
John C.S. Harding, Crissie Baker, Carrie Rhodes, Kathleen A. McIntosh, and Martin Bonneau
Two laboratory studies involving 11 laboratories were undertaken to assess the performance of North American Porcine circovirus-2 (PCV-2) polymerase chain reaction (PCR) assays. Laboratories received identical submissions containing randomly coded positive...
Published Jan 2009: Veterinary Microbiology Volume 133, Issues 1-2, 1 January 2009, Pages 54-64
Porcine Circovirus Type 2 in Muscle and Bone Marrow is Infectious and Transmissible to Naïve Pigs by oral consumption
Tanja Opriessnig, Abby R. Patterson, Xiang-Jin Meng and Patrick G. Halbur
Pork products are a possible source of introduction of PCV2 isolates into a pig population. However, limited work has been done on the transmission through meat of porcine circovirus type 2 (PCV2), a virus associated with several disease syndromes in...
Published Dec 2008: Vet Immunol Immunopathol. 2008 Dec 25. [Epub ahead of print]
Development of cell-mediated immunity to porcine circovirus type 2 (PCV2) in caesarean-derived, colostrum-deprived piglets.
Fort M, Fernandes LT, Nofrarias M, Díaz I, Sibila M, Pujols J, Mateu E, Segalés J.
The interaction between porcine circovirus type 2 (PCV2) and the pig immune system has been suggested to be a determinant event for the pathogenesis of postweaning multisystemic wasting syndrome (PMWS). To gain insight into the host immune mechanisms...
Published Dec 2008: The Veterinary Record, Dec 2008; 163: 737 - 740.
Efficacy of Immunising Pigs Against Porcine Circovirus Type 2 at Three or Six Weeks of Age
G. Cline, V. Wilt, E. Diaz, and R. Edler
The efficacy of a porcine circovirus type 2 (PCV-2) vaccine was tested in pigs vaccinated at three or six weeks of age. A total of 1106 weaned pigs were randomly allocated to one of three treatment groups: vaccinated at three weeks of age, vaccinated...
Published Dec 2008: Virus Res. 2008 Dec 15.
Classification and putative origins of Brazilian porcine circovirus 2 inferred through phylogenetic and phylogeographical approaches.
Chiarelli-Neto O, Yotoko KS, Vidigal PM, Silva FM, Castro LA, Fietto JL, Silva A Jr, Almeida MR.
Porcine circovirus 2 (PCV2) is the major causative agent of postweaning multisystemic wasting syndrome (PMWS) and is associated with different syndromes affecting pigs. The PCV2 genome has three main open reading frames (ORFs) among which the ORF2 encodes...
Published Dec 2008: Am J Vet Res. 2008 Dec;69(12):1608-14
Effect of coinfection with genogroup 1 porcine torque teno virus on porcine circovirus type 2-associated postweaning multisystemic wasting syndrome in gnotobiotic pigs.
Ellis JA, Allan G, Krakowka S.
Objective-To determine whether genogroup 1 porcine torque teno virus (g1-TTV) can potentiate clinical disease associated with porcine circovirus type 2 (PCV2). Sample population-33 gnotobiotic baby pigs. Procedures-Pigs were allocated into 7 groups: group...
Published Dec 2008: Can Vet J 2008;49:1189–1194
Quantitative polymerase chain reaction for Porcine circovirus-2 in swine feces in a Porcine circovirus disease-affected commercial herd and a nonaffected commercial herd
Kathleen A. McIntosh, John C.S. Harding, Sarah Parker, Steven Krakowka, Gordon Allan, John A. Ellis
This study examined if pigs in a Porcine circovirus disease (PCVD)-affected herd (n = 100) had shed more Porcine circovirus-2 (PCV-2) in their feces than pigs in a PCVD-nonaffected herd (n = 101), and if differences in shedding among production...
Published Nov 2008: The Veterinary Record, Nov 2008; 163: 536 - 538.
Lack of Transmission of Porcine Circovirus Type 2 to Weanling Pigs by Feeding them Spray-Dried Porcine Plasma
J. Pujols, S. López-Soria, J. Segalés, M. Fort, M. Sibila, R. Rosell, D. Solanes, L. Russell, J. Campbell, J. Crenshaw, E. Weaver, and J. Polo
An experiment was conducted to determine whether spray-dried porcine plasma containing 2·47 x 105 DNA copies of porcine circovirus type 2 (PCV-2) could infect weanling pigs when fed to them. Five specific pathogen-free (SPF) weanling pigs were...
Published Nov 2008: Vet Pathol. 2008 Nov;45(6):842-8
eproduction of postweaning multisystemic wasting syndrome in pigs by prenatal porcine circovirus 2 infection and postnatal porcine parvovirus infection or immunostimulation.
Ha Y, Lee YH, Ahn KK, Kim B, Chae C.
Postweaning multisystemic wasting syndrome (PMWS) was reproduced in prenatally porcine circovirus 2 (PCV2)-infected pigs by either postnatal infection with porcine parvovirus (PPV) or by immunostimulation. Twenty-four randomly selected piglets from 3...
Published Nov 2008: Vet Microbiol. 2008 Nov 5
A New Emerging Genotype Subgroup within PCV-2b Dominates the PMWS Epizooty in Switzerland.
Wiederkehr DD, Sydler T, Buergi E, Haessig M, Zimmermann D, Pospischil A, Brugnera E, Sidler X.
Postweaning multisystemic wasting syndrome (PMWS) is among the most important emerging pig diseases worldwide. Initially, the insidious nature of the disease made it difficult to pinpoint the pathogen. The presence of porcine circovirus type 2 (PCV2)...
Published Nov 2008: Veterinary Microbiology, 132 (1), p.19-28, Nov 2008
Hepatitis E virus infection dynamics and organic distribution in naturally infected pigs in a farrow-to-finish farm
de Deus N, Casas M, Peralta B, Nofrarias M, Pina S, Martin M, Segales J
The objective of the present study was to determine the pattern of Hepatitis E virus (HEV) infection in a naturally infected, farrow-to-finish herd. For that purpose, a prospective study was conducted in randomly selected 19 sows and 45 piglets. Blood...
Published Nov 2008: Veterinary Microbiology, 132 (1), p.165-170, Nov 2008
Detection of porcine circovirus type 2 in pigs imported from Indonesia
Manokaran G, Lin Y N, Soh M L, Lim E A S, Lim C W, Tan B H.
We have detected the presence of porcine circovirus (PCV) type 2 in Indonesian pigs imported to Singapore for food consumption. A total of three viral isolates were identified, and to genetically characterise them further, their full genomes were sequenced....
Published Nov 2008: Veterinary Microbiology, 132 (1), p.74-86, Nov 2008
Increased porcine circovirus type 2 replication in porcine leukocytes in vitro and in vivo by concanavalin A stimulation
Lefebvre D J, Meerts, P, Costers S, Misinzo G, Barbe F, Van Reeth K, Nauwynck H J
Previously, it was shown that modulation of the immune system enhances porcine circovirus type 2 (PCV2) replication in pigs. In the present study, the effect of the mitogen concanavalin A (ConA) on PCV2 replication was investigated. Since ConA induces...
Published Oct 2008: Vet Microbiol. 2008 Oct 17.
Infection, excretion and seroconversion dynamics of porcine circovirus type 2 (PCV2) in pigs from post-weaning multisystemic wasting syndrome (PMWS) affected farms in Spain and Denmark.
Grau-Roma L, Hjulsager CK, Sibila M, Kristensen CS, López-Soria S, Enøe C, Casal J, Bøtner A, Nofrarías M, Bille-Hansen V, Fraile L, Baekbo P, Segalés J, Larsen LE.
Longitudinal case-control studies were performed in post-weaning multisystemic wasting syndrome (PMWS) affected farms from Denmark and Spain using similar designs. Fourteen independent batches of 100-154 pigs per batch were monitored from birth to PMWS...
Published Oct 2008: J. Gen. Virol., Oct 2008; 89: 2482 - 2491
Differences in virulence among porcine circovirus type 2 isolates are unrelated to cluster type 2a or 2b and prior infection provides heterologous protection
T. Opriessnig, S. Ramamoorthy, D. M. Madson, A. R. Patterson, N. Pal, S. Carman, X. J. Meng and P. G. Halbur1
Porcine circovirus type 2 (PCV2) is divided into two genetic clusters designated PCV2a and PCV2b. The objectives of this study were to determine whether isolates from different clusters vary in virulence and to determine whether infection with PCV2a isolates...
