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PMWS Research Archives
Published Oct 2008: J Wildl Dis. 2008 Oct;44(4):864-70Detection and genetic characterization of porcine circovirus 2 isolates from the first cases of postweaning multisystemic and wasting syndrome in wild boars in Greece.
Sofia M, Billinis C, Psychas V, Birtsas P, Sofianidis G, Leontides L, Knowles N, Spyrou V.
In 2002, postweaning multisystemic wasting syndrome (PMWS) was diagnosed in a European female wild boar (Sus scrofa), based on the detection of porcine circovirus 2 (PCV2) DNA in various organs, including the uterus, and on histopathologic lesions. This...
Published Sep 2008: J Virol Methods. 2008 Sep 20. (Epub ahead of print)
Development of two Trichoplusia ni larvae-derived ELISAs for the detection of antibodies against replicase and capsid proteins of porcine circovirus type 2 in domestic pigs.
Pérez-Martín E, Grau-Roma L, Argilaguet JM, Nofrarías M, Escribano JM, Gómez-Sebastián S, Segalés J, Rodríguez F.
The main aim of the present study was to describe new methods for the identification of antibodies against the PCV2 capsid (Cap) and replicase (Rep) proteins in pig sera. Specifically, two new indirect enzyme-linked immunosorbent assays (ELISA) were developed...
Published Sep 2008: Vet Pathol. 2008 Sep;45(5):640-4.
Porcine circovirus 2 inclusion bodies in pulmonary and renal epithelial cells.
Huang YY, Walther I, Martinson SA, López A, Yason C, Godson DL, Clark EG, Simko E.
Porcine circovirus 2 (PCV2) is the cause of postweaning multisystemic wasting syndrome (PMWS). The most common lesions of PMWS are lymphohistiocytic to granulomatous lymphadenitis, interstitial pneumonia and interstitial nephritis, with intracytoplasmic...
Published Sep 2008: United States Patent and Trademark Office Granted Patent, Sep 2008 patno:US7425444
Circovirus sequences associated with piglet weight loss disease (PWD)
Jestin André, Albina Emmanuel, Le Cann Pierre, Blanchard Philippe, Hutet Evelyne, Arnauld Claire, Truong Catherine, Madec François
The genome sequences and the nucleotide sequences coding for the PWD circovirus polypeptides, such as the circovirus structural and non-structural polypeptides, vectors including the sequences, and cells and animals transformed by the vectors are provided....
Published Sep 2008: Transboundary and Emerging Diseases - Volume 55, Issue 7, Date: September 2008, Pages: 273-283
Post-Weaning Multisystemic Wasting Syndrome and Other PCV2-Related Problems in Pigs: a 12-Year Experience
F. Madec, N. Rose, B. Grasland, R. Cariolet, A. Jestin
Post-weaning multisystemic wasting syndrome (PMWS) and other porcine circovirus type 2 (PCV2)-related diseases have been reported throughout the world for about 10 years. The present paper reviews the knowledge acquired in different fields and is largely...
Published Sep 2008: Journal of Virological Methods, Volume 152, Issues 1-2, September 2008, Pages 112-116
Rolling-circle amplification for the detection of active porcine circovirus type 2 DNA replication in vitro
Paolo Dominic Navidad, Hao Li, Annette Mankertz, Brian Meehan
Porcine circovirus type 2 (PCV2) infections in pigs have diverse clinical presentations and are considered economically important diseases worldwide. However, despite intensive research, the early pathogenesis of PCV2 and the primary target cells for...
Published Sep 2008: United States Patent and Trademark Office Pre-grant Publication, Sep 2008 patno:US20080233147
Circovirus sequences associated with piglet weight loss disease (PWD)
Jestin Andre, Albina Emmanuel, Le Cann Pierre, Blanchard Philippe, Hutet Evelyne, Arnauld Claire, Truong Catherine, Madec Francois
The genome sequences and the nucleotide sequences coding for the PWD circovirus polypeptides, such as the circovirus structural and non-structural polypeptides, vectors including the sequences, and cells and animals transformed by the vectors are provided....
Published Aug 2008: Research in Veterinary Science Volume 85, Issue 1, August 2008, Pages 197-200
Molecular diversity of Brazilian strains of porcine circovirus type 2 (PCV-2)
Alessandra Marnie Martins Gomes de Castro, Adriana Cortez, Marcos Bryan Heinemann, Paulo Eduardo Brandão, and Leonardo José Richtzenhain
Porcine circovirus 2 (PCV-2) is associated with a broad range of syndromes. In this study, eight pig tissue samples from two Brazilian states were analyzed using six PCR primer pairs amplifying a 1705-bp fragment of the PCV-2 genome. The NJ distance-based...
Published Aug 2008: J. Gen. Virol., Aug 2008; 89: 1857 - 1865.
Protective immunity against porcine circovirus 2 by vaccination with ORF2-based DNA and subunit vaccines in mice
Hui-Gang Shen, Ji-Yong Zhou, Zhen-Yu Huang, Jun-Qing Guo, Gang Xing, Jia-Ling He, Yan Yan and Li-Yang Gong
The protective immune response against porcine circovirus 2 (PCV2) infection in mice was characterized using flow cytometric analysis (FCM), assays of antibody (of different IgG isotypes) and viraemia, and histopathological examination. An open reading...
Published Aug 2008: Vet Immunol Immunopathol. 2008 Aug 15;124(3-4):355-66. Epub 2008 May 4.
Characterization of porcine circovirus type 2 (PCV2) infection in swine lymphocytes using mitogen-stimulated peripheral blood lymphocytes from healthy PCV2-carrier pigs.
Lin CM, Jeng CR, Chang HW, Guo IC, Huang YL, Tsai YC, Chia MY, Pang VF.
Information regarding the susceptibility of swine lymphocytes to PCV2 is rather limited. To further explore and characterize the PCV2 infection in swine lymphocytes, an in vitro model using concanavalin A (Con A)-stimulated peripheral blood lymphocytes...
Published Aug 2008: Vaccine, 26 (33), p.4231-4236, Aug 2008
A genetically engineered chimeric vaccine against porcine circovirus type 2 (PCV2) is genetically stable in vitro and in vivo
J. Gillespie, N.M. Juhan, J. DiCristina, K.F. Key, S. Ramamoorthy and X.J. Meng
A vaccine against porcine circovirus type 2 (PCV2), designated PCV1-2 chimera, was recently developed by replacing the capsid gene of the non-pathogenic PCV1 with that of PCV2. The PCV1-2 chimera virus is attenuated in pigs but induces protective immunity...
Published Jul 2008: Veterinary Immunology and Immunopathology Volume 124, Issues 1-2, 15 July 2008, Pages 41-49
Porcine circovirus type 2 (PCV2) viral components immunomodulate recall antigen responses
Tuija Kekarainen, Maria Montoya, Javier Dominguez, Enric Mateu, and Joaquim Segalés
Porcine circovirus type 2 (PCV2) is a single-stranded circular DNA virus infecting domestic pigs worldwide. Interaction of this virus with the immune system apparently modulates the immune response of the host. In the present study, the implication of...
Published Jul 2008: Research in Veterinary Science, In Press, Corrected Proof, Available online 10 July 2008
A proteomic reference map for pig serum proteins as a prerequisite for diagnostic applications
Ingrid Miller, Robin Wait, Wolfgang Sipos, Manfred Gemeiner
A reference protein map for pig serum was set up using two-dimensional electrophoresis (2-DE). Thirty-nine protein chains or spots deriving from 26 different proteins were identified by immunological and mass spectrometric methods. Thus, the positions...
Published Jul 2008: Vet Microbiol. 2008 Jul 4.
Inter-laboratory and inter-assay comparison on two real-time PCR techniques for quantification of PCV2 nucleic acid extracted from field samples.
Hjulsager CK, Grau-Roma L, Sibila M, Enøe C, Larsen L, Segalés J.
Several real-time PCR assays for quantification of PCV2 DNA (qPCR) have been described in the literature, and different in-house assays are being used by laboratories around the world. A general threshold of 10(7) copies of PCV2 per millilitre serum for...
Published Jul 2008: Vet Microbiol. 2008 Jul 7
A meta-analysis on experimental infections with porcine circovirus type 2 (PCV2).
Tomás A, Fernandes LT, Valero O, Segalés J.
A meta-analysis was performed with the aim to identify factors with a relevant influence on the expression of clinical postweaning multisystemic wasting syndrome (PMWS) under experimental conditions. Data from 44 studies were included in the analysis....
