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PMWS Research Archives

Published Nov 2011: J Vet Diagn Invest. 2011 Nov;23(6):1160-7. Epub 2011 Oct 24.
Comparative Evaluation of a Competitive Polymerase Chain Reaction (PCR) and a SYBR Green-based Real-time PCR to Quantify Porcine circovirus-2 DNA in Swine Tissue Samples.
Dezen D, Rijsewijk FA, Teixeira TF, Holz CL, Varela AP, Cibulski SP, Gregianini TS, Batista HB, Franco AC, Roehe PM.
Porcine circovirus-2 (PCV-2) is considered the major etiological agent of post-weaning multisystemic wasting syndrome (PMWS) in pigs. The clinical manifestations of the disease are correlated with moderate to high amounts of PCV-2 DNA in biological samples...

Published Nov 2011: Journal of Virological Methods - In Press, Uncorrected Proof
A Multiple SYBR Green I-Based Real-Time PCR System for the Simultaneous Detection of Porcine Circovirus Type 2, Porcine Parvovirus, Pseudorabies Virus and Torque Teno Sus Virus 1 and 2 in Pigs
Lester J. Pérez, Carmen L. Perera, Maria T. Frías, José I.Núñez, LliliannE Ganges, Heidy Díaz de Arce
Multiple viral infections are common in pigs under intensive production conditions. All five of the viruses included in this study are associated with multifactorial diseases that cause significant economic losses in swine farming worldwide. The development...

Published Nov 2011: Virology Journal 2011, 8:517
Co-Existence of Multiple Strains of Porcine Circovirus Type 2 in the same Pig from China
Shao-Lun Zhai, Sheng-Nan Chen, Zu-Zhang Wei, Jian-Wu Zhang, Lv Huang, Tao Lin, Cheng Yue, Duo-Liang Ran, Shi-Shan Yuan, Wen-Kang Wei and Jin-Xue Long
Pigs are often co-infected by different viral strains from the same virus. Up to now, there are few reports about co-existence of different porcine circovirus type 2 (PCV2) strains in China. The aim of this study was to evaluate it in Chinese swine herds....

Published Nov 2011: Virus Res. 2011 Nov 9. [Epub ahead of print]
A Nine-Base Nucleotide Sequence in the Porcine Circovirus Type 2 (PCV2) Nucleocapsid Gene Determines Viral Replication and Virulence.
Krakowka S, Allan G, Ellis J, Hamberg A, Charreyre C, Kaufmann E, Brooks C, Meehan B.
Porcine circovirus type 2 (PCV2) was retrospectively identified by serology in swine populations as an asymptomatic infection at least 25 years prior to the first reported case of PCV2-associated postweaning multisystemic wasting syndrome (PMWS). To investigate...

Published Nov 2011: Journal of Virological Methods - In Press, Uncorrected Proof
Use of PCR-Based Assays for the Detection of the Adventitious Agent Porcine Circovirus Type 1 (PCV1) in Vaccines, and for Confirming the Identity of Cell Substrates and Viruses used in Vaccine Production
Deepak Kumar, Nathan M. Beach, Xiang-Jin Meng, Nagendra R. Hegde
Safety and quality are important issues for vaccines. Whereas reversion to virulence poses a safety risk with live attenuated vaccines, the potential for the presence of adventitious agents is also an issue of vaccine quality. The recent detection or...

Published Nov 2011: Virus Res. 2011 Nov 11. [Epub ahead of print]
Cell Tropism and Entry of Porcine Circovirus 2.
Nauwynck HJ, Sanchez R, Meerts P, Lefebvre DJ, Saha D, Huang L, Misinzo G.
Porcine circovirus 2 (PCV2) may induce reproductive failure (return to oestrus, embryonic death, mummification, weak- and stillborn piglets) and postweaning multisystemic wasting syndrome (PMWS). Furthermore, it may modulate the immunity in such a way...

Published Nov 2011: Virol J. 2011 Nov 2;8(1):497. [Epub ahead of print]
Loop-Mediated Isothermal Amplification for Detection of Porcine Circovirus Type 2.
Zhou S, Han S, Shi J, Wu J, Yuan X, Cong X, Xu S, Wu X, Li J, Wang J.
BACKGROUND: Porcine circovirus type 2 (PCV2) is the primary causative agent of the emerging swine disease known as postweaning multisystemic wasting syndrome (PMWS). Nowadays, polymerase chain reaction (PCR) is still the most widespread technique in pathogen...

Published Nov 2011: J. Virol. November 2011; published ahead of print 24 August 2011
Coreplication of the Major Genotype Group Members of Porcine Circovirus Type 2 as a Prerequisite to Coevolution May Explain the Variable Disease Manifestations
Siegfried Khaiseb, Titus Sydler, Dieter Zimmermann, Andreas Pospischil, Xaver Sidler, and Enrico Brugnera
A member of the family Circoviridae, porcine circovirus type 2 (PCV2), is associated with postweaning multisystemic wasting syndrome (PMWS), a recent emerging disease worldwide. PCV2 is also found in clinically asymptomatic animals. This paradoxical finding...

Published Oct 2011: Virus Res. 2011 Oct 20. [Epub ahead of print]
Porcine Circovirus Type 2 and its Associated Diseases in Korea.
Chae C.
This review describes the characterization of porcine circovirus type 2 (PCV2), the field situation of porcine circovirus-associated disease (PCVAD) and the PCV2 vaccine in Korea. PCVAD has been considered the most devastating disease in Korean livestock...

Published Oct 2011: Virus Res. 2011 Oct 15. [Epub ahead of print]
Porcine Circovirus Type 2 and its Associated Diseases in China.
Ge X, Wang F, Guo X, Yang H.
Porcine circovirus type 2 (PCV2) has been recognized as an important vial pathogen for global swine industry. The virus is associated with postweaning multisystemic wasting syndrome (PMWS) and other syndrome diseases collectively known as porcine circovirus-associated...

Published Oct 2011: Arch Virol. 2011 Oct 2. [Epub ahead of print]
Genetic Characterization and Phylogenetic Analysis of Porcine Circovirus Type 2 (PCV2) in Serbia.
Savic B, Milicevic V, Jakic-Dimic D, Bojkovski J, Prodanovic R, Kureljusic B, Potkonjak A, Savic B.
Porcine circovirus type 2 (PCV2) is the main causative agent of postweaning multisystemic wasting syndrome (PMWS). To characterize and determine the genetic diversity of PCV2 in the porcine population of Serbia, nucleotide and deduced amino acid sequences...

Published Sep 2011: Veterinary Record 2011; Volume 169, Issue 13 September
Severity of PMWS: Investigating Risk Factors on Farms
P. Alarcon, M. Velasova, A. Mastin, A. Nevel, K. D. C. Stärk, B. Wieland
This study identified risk factors associated with different levels of severity of postweaning multisystemic wasting syndrome (PMWS) on pig farms in England. During 2008/09, 147 farms across England were studied. They were recruited through a porcine...

Published Sep 2011: Veterinary Microbiology - Volume 152, Issues 3-4, 28 September 2011, Pages 235-246
Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Influences Infection Dynamics of Porcine Circovirus Type 2 (PCV2) Subtypes PCV2a and PCV2b by Prolonging PCV2 Viremia and Shedding
A. Sinha, H.G. Shen, S. Schalk, N.M. Beach, Y.W. Huang, X.J. Meng, P.G. Halbur, T. Opriessnig
The objective of this study was to determine the effect of porcine reproductive and respiratory syndrome virus (PRRSV) infection on porcine circovirus type 2 (PCV2) subtypes a (PCV2a) or b (PCV2b) viremia and shedding characteristics in oral, nasal and...

Published Sep 2011: Vaccine - Available online 21 September 2011
Construction and Immunogenicity of Recombinant Adenovirus Expressing ORF2 of PCV2 and Porcine IFN Gamma
Liu Genmei, Luo Manlin, Chen Ruiai, Huang Hongliang, Pei Dangshuai
The capsid structural protein encoded by the gene ORF2 of Porcine Circovirus Type 2 is critical for eliciting the broad protective immunity. We constructed and characterized the recombinant adenovirus encoding the capsid and porcine IFN gamma, designated...

Published Sep 2011: Virus Research - Available online 16 September 2011.
Discovery and Evolving History of Two Genetically Related but Phenotypically Different Viruses, Porcine Circoviruses 1 and 2
Gordon Allana, Steven Krakowka, John Ellis, Catherine Charreyre
Porcine circoviruses (PCVs) belong to the genus Circovirus, family Circoviridae, and are the smallest non-enveloped, single stranded, negative sense, circular DNA viruses that replicate autonomously in mammalian cells. Two types of PCV have been characterised,...

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