Salmonella Detection Methods in Feeds Compared07 April 2009
SWEDEN - Koyuncu and Haggblom at the National Veterinary Institute in Uppsala found that the accuracy, sensitivity and specificity of the three cultural methods they compared were similar although the the detection levels for different feed and feed ingredients varied considerably.
Animal feed as a source of infection to food producing animals is much debated. In order to increase our present knowledge about possible feed transmission it is important to know that the present isolation methods for Salmonella are reliable also for feed materials.
In a comparative study the ability of the standard method used for isolation of Salmonella in feed in the Nordic countries, the NMKL71 method (Nordic Committee on Food Analysis) was compared to the Modified Semisolid Rappaport Vassiliadis method (MSRV) and the international standard method (EN ISO 6579:2002).
Five different feed materials were investigated, namely wheat grain, soybean meal, rapeseed meal, palm kernel meal, pellets of pig feed and also scrapings from a feed mill elevator.
Four different levels of the Salmonella serotypes – S. Typhimurium, S. Cubana and S. Yoruba – were added to each feed material.
For all methods, pre-enrichment in Buffered Peptone Water (BPW) were carried out followed by enrichments in the different selective media and finally plating on selective agar media.
The results obtained with all three methods showed no differences in detection levels, with an accuracy and sensitivity of 65 per cent and 56 per cent, respectively. However, Müller-Kauffmann tetrathionate-novobiocin broth (MKTTn), performed less well due to many false negative results on Brilliant Green agar (BGA) plates. Compared to other feed materials, palm kernel meal showed a higher detection level with all serotypes and methods tested.
The results of this study showed that the accuracy, sensitivity and specificity of the investigated cultural methods were equivalent. However, the detection levels for different feed and feed ingredients varied considerably.
Koyuncu S. and P. Haggblom, 2009. A comparative study of cultural methods for the detection of Salmonella in feed and feed ingredients. BMC Veterinary Research 2009, 5:6doi:10.1186/1746-6148-5-6
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