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Information received on 24/01/2007 from Mr Tibor Bálint, Chief Veterinary Officer, Animal Health and Food Control Department, Ministry of Agriculture and Rural Development , Budapest, Hungary

Summary

Report type	Immediate notification
Start date	10/01/2007
Date of confirmation of event	22/01/2007
Report date	24/01/2007
Date submitted to OIE	25/01/2007
Reason for notification	Reoccurrence of listed disease
Date of previous occurrence	05/1993
Manifestation of disease	Clinical disease
Causal agent	Classical swine fever virus
Nature of diagnosis	Suspicion, Clinical, Laboratory (basic), Laboratory (advanced)
Report pertains to	Entire country

New Outbreaks

Outbreak 1	Csesztve, Balassagyarmat, NOGRAD
Date of start of outbreak	10/01/2007
Outbreak status	Continuing (or date resolved not submitted)
Epidemiological unit	Not applicable
Affected animals	Species Susceptible Cases Deaths Destroyed Slaughtered Wild species 1071 1 0 1 0
Affected population	wild boar
Outbreak 2	Ipolytarnóc, Salgótarján, NOGRAD
Date of start of outbreak	12/01/2007
Outbreak status	Continuing (or date resolved not submitted)
Epidemiological unit	Not applicable
Affected animals	Species Susceptible Cases Deaths Destroyed Slaughtered Wild species 1071 1 0 1 0
Affected population	wild boar
Outbreak 3	Litke, Salgótarján, NOGRAD
Date of start of outbreak	10/01/2007
Outbreak status	Continuing (or date resolved not submitted)
Epidemiological unit	Not applicable
Affected animals	Species Susceptible Cases Deaths Destroyed Slaughtered Wild species 1071 1 0 1 0
Affected population	wild boar
Summary of outbreaks	Total outbreaks: 3
Total animals affected	Species Susceptible Cases Deaths Destroyed Slaughtered Wild species 3213 3 0 3 0
Outbreak statistics	Species Apparent morbidity rate Apparent mortality rate Apparent case fatality rate Proportion susceptible removed* Wild species 0.09% 0.00% 0.00% 0.09% * Removed from the susceptible population either through death, destruction or slaughter

Epidemiology

Source of infection

Contact with wild Species

Epidemiological comments

The first, a clinically healthy wild boar, was shot near Csesztve on 10 January 2007. This animal was found to be positive on serological and virological (PCR) investigation. The second, a clinically healthy wild boar, was shot near Litke on 10 January 2007. The blood sample from this animal was unfit for serological investigation, but the sample from the tonsils was virologically (PCR) positive. The third was a wild boar shot near Ipolytarnóc on 12 January 2007 because of abnormal behaviour with neurological signs. The whole carcass was sent to the Veterinary Diagnostic Directorate in Budapest (the former Central Veterinary Institute), where the virological investigations (antigen capture ELISA and PCR) were positive. The positive samples and, for comparative reasons, a vaccine C strain were amplified by PCR based on the protocol described by Elvander et al. in 1998 (Acta vet. Scand. Vol. 39, pages 251-264). The amplified products (partial 5’UTR fragment, 188 base pairs) were purified from agarose gels and sequenced with the primers used in PCR. Based on alignments and BLAST homology search results, we were able to determine that: - The field sample sequences are identical but differ in 11 nucleotide positions compared with the vaccine strain in the examined region (the vaccine strain was 100% identical to the vaccine strain available in the GenBank). - The greatest homology was found with the Swiss isolate “Switzerland 4/93“ (3 nucleotide difference) accession number: AF045072 described as similar to the Alfort/187 strain. The results are preliminary, in order to elucidate the importance of the mutations or sequence differences, and we will perform two other PCR tests targeting the 3’ UTR and E2 regions.

Control measures

Measures already applied	Screening Control of wildlife reservoirs Zoning Vaccination prohibited No treatment of affected animals
Measures to be applied	None specified

Diagnostic test results

Laboratory name and type	Veterinary Diagnostic Directorate of the Central Agricultural Office, Budapest (former Central Veterinary Institute) (National laboratory)
Tests and results	Species Test Test date Result Wild species antigen (Ag) detection ELISA 22/01/2007 Positive Wild species polymerase chain reaction (PCR) 22/01/2007 Positive

Map of outbreak locations

Location of current outbreaks

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