Processing fluids provide an option to monitor PCV2 and PCVAD

Porcine circovirus type 2 (PCV2) is found among pig herds throughout the world and contributes to the disease syndrome known as porcine circovirus associated disease (PCVAD). Upping the challenge to addressing this ubiquitous virus is the fact that it is resilient to many common disinfectants, which allows it to remain and circulate within the pigs’ environment.
calendar icon 18 May 2020
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All of the farms had identified PCV2 clinical disease during general diagnostic submissions in the year prior to this study, Frealy said. However, a low proportion of PCR-positive processing-fluid samples was detected. “Considering the ubiquity of PCV2, these results are remarkable, as processing fluids are considered a highly sensitive test,” she added.

Overall, these results are consistent with previous research that recommended continued PCVAD monitoring downstream when processing-fluid PCR Ct (cycle threshold) values are lower than 30. Conversely, this study showed that when processing-fluid Ct values were above 30, little PCVAD was observed in the nurseries.

The low proportion of PCV2 detected by qPCR in processing fluids and in serum samples may be associated with the vaccination protocols within these farms, Frealy noted. “Due to the variable proportion of IFA titers detected across farms, further evaluation is warranted,” she concluded.

1 Frealy K, et al.
(2020) Evaluating the use of processing fluids for sow herd monitoring of porcine circovirus type 2. Student Research Posters.. Am Assoc. Swine Vet Annual Meeting. 2020;249.

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