ABSTRACTS

SECTION 1: VIRUS HETEROGENEITY
The challenge of hiv global diversity for vaccine antigen design - B Korber Theoretical Biology and Biophysics, Los Alamos National Laboratory, Los Alamos NM

Identification of the binding domain of sialoadhesin on macrophage cells to PRRSV - T-Q An, Y-J Zhou, Y-X He, Z-J Tian, H-J, Qiu, G-Z Tong* National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China

Essential and nonessential regions of PRRSV nsp2 - J Han, Y Wang, KS Faaberg* College of Veterinary Medicine, University of Minnesota

PRRSV glycoproteins: Mutation, expression and functional characterization - H Liu, Y Wang, J Han, KS Faaberg* College of Veterinary Medicine, University of Minnesota

Characterization of the North American (NA) and European (EU) PRRS viruses found in a co-infected pig in Hong Kong - 5 VYY Li, CKW Wong, FCC Leung* Department of Zoology, The University of Hong Kong, Hong Kong Special Administrative Region (HKSAR), China

PRRSV: Diversity of strains found in Britain - JP Frossard*, D Westcott, B Naidu, NGA Woodger, T Drew Veterinary Laboratories Agency (Weybridge), New Haw, Addlestone, Surrey KT15 3NB, UK

Evolutionary biology of PRRS viruses in pigs: Mutations and recombination - S-H Cha1, C-C Chang2, W-I Kim1, R Molina1, JJ Zimmerman1, K-J Yoon1* 1College of Veterinary Medicine, Iowa State University 2Department of Veterinary Medicine, National Chia-Yi University, Chunan, Miaoli, Taiwan

Evolutionary dynamics of North American-type PRRSV into three subtypes characterized by two deletional mutations in nsp2 gene - M Yoshii1*, A Miyazaki1, K Kato2, H Ikeda2, T Okinaga1, H Tsunemitsu1 1Research Team for Viral Diseases, 2Research Team for Zoonotic Diseases, National Institute of Animal Health, Tsukuba, Japan

Infectious cDNA clones for PRRSV - Past, present, and future - D Yoo Department of Pathobiology, Ontario Veterinary College, University of Guelph

Increased viremia, altered immune responses, fever, and pathologic lesions following intranasal heterologous wild-type PRRSV challenge: Identification of markers associated with incomplete cross-protection in a respiratory disease model - MB McCaw1*, J Lunney2, W Tompkins1 1College of Veterinary Medicine, North Carolina State University 2Animal Parasitic Diseases Laboratory, ANRI, ARS, USDA, Beltsville, MD

Localization properties of PRRSV nonstructural protein 2 (nsp2) - D-Y Kim1, M Kerrigan1, JG Calvert2, RRR Rowland1 1Department of Diagnostic Medicine and Pathobiology, Kansas State University 2Pfizer Animal Health, Kalamazoo, MI

SECTION 2: VIRAL DIAGNOSTICS
Development of an optical-based nanobiosensor for detection of PRRSV - RC Stringer1*, S Grant1, S Schommer2, D Hoehn1 1Department of Biological Engineering, 2Department of Veterinary Pathobiology, University of Missouri-Columbia

Low voltage capillary electrophoresis of 750 bp PRRSV isolates on a microchip - D Gangopadhyay2, S Bhattacharya1*, V Korampally3, SB Kleiboeker4, SA Grant5, K Gangopadhyay3, S Gangopadhyay3 1Birck Nanotechnology Center, Purdue University 2Rock Bridge High School, Columbia, MO 3Department of Electrical and Computer Engineering, University of Missouri-Columbia, 4Viracor Laboratories, Kansas City, KA 5Department of Biological Engineering, University of Missouri-Columbia

Detection of PRRSV infection using pen-based oral fluid samples: Experimental study - J Prickett1*, R Simer2, K-J Yoon1, J Zimmerman1 1College of Veterinary Medicine, Iowa State University 2Simer Veterinary Services, Perryton, TX

Detection of PRRSV infection using pen-based oral fluid samples: Field study - J Prickett1*, R Simer2, W Kim1, K-J Yoon1, J Zimmerman1 1College of Veterinary Medicine, Iowa State University 2Simer Veterinary Services, Perryton, TX

Evaluation of the cysteine protease domain of PRRSV nsp2 as a potential new antigen for ELISA development - J Schwartz1*, N Benson1, B Neiger1, HX Liu1, RR Rowland2, DA Benfield3, J Christopher-Hennings1, EA Nelson1, Y Fang1 1Dept of Veterinary Science, Center for Infectious Disease Research and Vaccinology, South Dakota State University 2Dept of Diagnostic Medicine and Pathobiology, Kansas State University 3Food Animal Health Research Program, Agricultural Research and Development Center, Wooster, OH

Development and optimization of a blocking ELISA for Type 1 and Type 2 strains of PRRSV - S Lawson1,2*, E Brown1, K Gilkerson2, Y Fang1, EA Nelson1, C Mateo2, C Chase1 1Department of Veterinary Science, South Dakota State University 2Rural Technologies Inc, Brookings, SD

Preparation of monoclonal antibodies against the non-structural proteins of PRRSV - B Berkenpas1*, M Murtaugh2, N Benson1, J Schwartz1, B Neiger1, E Nelson1, Y Fang1 1Department of Veterinary Science, South Dakota State University 2Department of Veterinary and Biomedical Sciences, University of Minnesota

PRRSV surveillance, elimination, and immunity in boars and boar semen - A Rovira1, T Clement2*, J Christopher-Hennings2, C Muñoz-Zanzi3, K Rossow4, N Loskutoff5, E Nelson2, B Thompson6, M Engle6, D Reicks7, M Murtaugh1, J Lunney8 1College of Veterinary Medicine, University of Minnesota 2Department of Veterinary Science, South Dakota State University 3Division of Epidemiology and Community Health, School of Public Health, University of Minnesota 4Minnesota Veterinary Diagnostic Laboratory, University of Minnesota 5Henry Doorly Zoo, Omaha, Nebraska 6PIC, North America, Hendersonville, TN 7Swine Vet Center, St. Peter, MN 8ANRI, ARS, USDA. BARC-East, Beltsville, MD

SECTION 3: IMMUNE AND GENETIC RESPONSE
Genome-wide transcriptional map of innate immune responses to replication of PRRSV in alveolar macrophages of commercial breeds - T Ait-Ali1*, A Wilson1, S Ganeshprasad1, D Wescott2, J-P Frossard2, M Waterfall1, M Clapperton1, M Mellencamp 3, TW Drew2, S Bishop1, A Archibald1 1Roslin institute, Edinburgh, UK 2VLA, Weybridge, UK 3Genus/PIC, Franklyn, KY

USP18 contributes to PRRSV antiviral response by repressing JAK-STAT signaling - T Ait-Ali1*, S Ramaiahgari1, N Reetoo1, A Joshi1, DG Wescott2, M Waterfall1, W Carré1, J-P Frossard2, KH Baek3, TW Drew2, SC Bishop1,AL Archibald1 1Roslin Institute, UK 2VLA, Weybridge, UK 3Cell and Gene Therapy Research Institute, Korea

Recombinant adenovirus expressing PRRSV minor envelope protein GP3 induces cellmediated and humoral immune responses in mice - WM Jiang, P Jiang* Key Laboratory of Animal Diseases Diagnostic and Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Ministry of Agriculture, Nanjing 210095, China

A porcine myelomonocytic cell line, 3D4/31 is susceptible to PRRSV infection in vitro - CKW Wong, VYY Li, CM Ma, FCC Leung* Department of Zoology, The University of Hong Kong, Hong Kong Special Administrative Region (HKSAR), China

Antibody response to the cysteine protease of PRRSV nsp2 - Y Fang1*, J Schwartz1, XF Gao1, N Benson1, RR Rowland2, DA Benfield3, E Nelson1 1Dept of Veterinary Science, Center for Infectious Disease Research and Vaccinology, South Dakota State University 2Dept of Diagnostic Medicine and Pathobiology, Kansas State University 3Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Wooster, OH

IgY neutralizing antibodies against PRRSV reduce viral movement in a positive farm unit - AT Francos*, HD Herrera, RC Chong, GA Morales, DE Lucio, A Rodríguez-Ropón Investigación Aplicada SA de CV Tehuacan Puebla, México

Protection of alveolar macrophages and MARC-145 cells from prrsv challenge by swine interferon-beta - C Overend1*, R Mitchell1, D He3, G Rompato1, MJ Grubman2, AE Garmendia1 1Department of Pathobiology and Veterinary Science, University of Connecticut 2Plum Island Animal Disease Center, ARS, USDA, Greenport, New York 3College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China

Presence of interferon-alpha delays viral replication and reduces disease signs in pigs challenged with PRRSV - SL Brockmeier1*, KM Lager1, MJ Grubman2, DE Brough3, D Ettyreddy3, RE Sacco1, PC Gauger1, HD Lehmkuhl1 1National Animal Disease Center, Ames, IA 2Plum Island Animal Disease Center, Greenport, NY 3 GenVec, Inc., Gaithersburg, MD

Do immune genes indicate which pigs will have persistent PRRSV infections? - J Lunney*1, RRR Rowland2, R Molina3, J Hermann3, D Kuhar1, J Christopher-Hennings4, E Nelson4, V Leathers5, J Zimmerman3 1USDA ARS BARC 2Kansas State University 3Iowa State University 4South Dakota State University 5IDEXX Laboratories, Inc., Westport ME

Passive immunization of young piglets with hyperimmune plasma containing virus neutralizing antibodies to PRRSV - LD Kaniewski1*, JD Hocker1,2, MA Mogler1, MM Erdman1, EA Nelson3, DL Harris 1,4,5 1Dept Animal Science, Iowa State University 2AMVC Management Services, Audobon, IA 3Dept Veterinary Science, South Dakota State University 4 Veterinary Diagnostic and Production Animal Medicine, Iowa State University 5Sirrah LLC, Ames, IA

Production of equine plasma containing high neutralizing antibody titers to PRRSV - MA Mogler1*, JD Hocker1,4, LD Kaniewski1, MM Erdman1, DL Harris 1,2,3 1Dept of Animal Science, Iowa State University, 2Dept of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, 3Sirrah LLC, Ames, IA, 4AMVC Management Services, Audubon, IA

Structural studies of arterivirus proteins - T Dokland*1, A Deshpande1, D Doan2, M Walsh3 1Department of Microbiology, University of Alabama at Birmingham 2Institute of Molecular and Cell Biology, Singapore 3European Synchrotron Radiation Facility, Grenoble, France

Presence of haptoglobin a1S subunit in serum is associated with acute PRRSV infection - JS Gnanandarajah*, C Dvorak, C Johnson, MP Murtaugh Department of Veterinary and Biomedical Sciences, University of Minnesota

The selected B cell population in PRRS has a naïve phenotype, undiversified repertoire, and unusually hydrophobic HCDR3 - JE Butler1, P Weber1, CD Lemke1, K Kimura2, M Sinkora3, A Vincent2, A Vorwald2, KM Lager2 1Department of Microbiology, University of Iowa 2National Animal Disease Center, USDA, ARS, Ames, IA 3Czech Academy of Science, Novi Hradek, Czech Republic

In vitro inhibition and enhancement of PRRSV infection by porcine seminal plasma is not due to the effects of complement - T Okinaga12*, M Yoshii2, H Tsunemitsu2, DJ Hurley1 1Department of Population Health, College of Veterinary Medicine, University of Georgia 2Research Team for Viral Disease, National Institute of Animal Health, Tsukuba, Japan

Absence of IRF-3 activation in PRRSV infection - F Lai*, D Yoo Department of Pathobiology, Ontario Veterinary College, University of Guelph

Role of structural proteins in cross neutralization among PRRS viruses - W-I Kim, S-H Cha, K-J Yoon* College of Veterinary Medicine, Iowa State University

Porcine humoral immune response to PRRSV GP5-M - J Li1*, R Molina3, RRR Rowland2, JJ Zimmerman3, C Johnson1, M Fuentes 1, M Roof4, G Haiwick4, E Nelson5, MP Murtaugh1 1Dept of Veterinary and Biomedical Sciences, University of Minnesota 2Dept of Diagnostic Medicine Pathobiology, Kansas State University 3College of Veterinary Medicine, Iowa State University 4Boehringer Ingelheim Vetmedica, Inc, Ames, IA 5Department of Veterinary Science, South Dakota University

Porcine monocyte- and skin-derived dendritic cells are susceptible to PRRSV infection, but cytokine production is differently modulated - J Hernández*, M Reséndiz, L López, L Flores, E Silva Laboratorio de Inmunología, Centro de Investigación en Alimentación y Desarrollo, AC, Hermosillo, Sonora, México

Mechanisms of cross-protective immunity against PRRSV - MP Murtaugh Department of Veterinary and Biomedical Sciences, University of Minnesota

Understanding mucosal immunity and disease resistance Volker Gerdts Vaccine and Infectious Disease Organization, University of Saskatchewan

Detection of T cell response after in vitro stimulation with GP5 peptides from PRRSV VR- 2332 isolate - CR Wyatt*, RRR Rowland Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University

Wild-type, as well as attenuated versions of PRRSV, selectively inhibit the ability of porcine plasmacytoid dendritic cells to produce interferon alpha - G Calzada-Nova*, RJ Husmann, WM Schnitzlein, FA Zuckermann Department of Pathobiology University of Illinois

SECTION 4: VACCINES
Blocking of PRRSV replication by Stealth RNAi in MARC-145 cells - V Lazar, R Pogranichniy Animal Disease Diagnostic Laboratory, Department of Veterinary Pathobiology, Purdue University

Comparative serological investigation of early piglet vaccination with Ingelvac® PRRS MLV to vaccination at weaning - M Genzow1*, G Schagemann2 1Boehringer Ingelheim Animal Health GmbH, Ingelheim Germany 2Boehringer Ingelheim Vetmedica GmbH

Replicon particle co-expression of PRRSV GP5 and M proteins - MM Erdman1*, KI Kamrud2, DL Harris 1,3,4 1Department of Animal Science, Iowa State University, 2AlphaVax Inc, Research Triangle Park, NC, 3Dept of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, 4Sirrah LLC, Ames, IA

Deceptive imprinting and immune refocusing - the next generation of discovery and development for vaccines - PL Nara, G Lin, G Tobin Biological Mimetics, Inc, Frederick, MD

Functional mapping of the PRRSV capsid protein nuclear localization signal and its pathogenic association - Y Pei1, D Hodgins1, C Lee1, JG Calvert*2, SK Welch2, R Jolie2, M Keith2, D Yoo1 1Dept of Pathobiology, Ontario Veterinary College, University of Guelph, 2Pfizer Animal Health, Kalamazoo, MI

Recombinant PRRSV expressing the porcine circovirus Type 2 capsid protein - immunogenicity in pigs - Y Pei1, D Hodgins1, J G Calvert2, S K Welch2, C Song1, D Yoo*1 1Dept of Pathobiology, Ontario Veterinary College, University of Guelph 2Pfizer Animal Health, Kalamazoo, MI

Immunogenicity and protection efficacy of a herpes simplex virus-based amplicon vector expressing codon-optimized glycoprotein 5 of PRRSV - M Eaton1, WJ Bowers2,3, HJ Federoff2,3,4, X Wang1,5* 1Department of Biology and Microbiology, South Dakota State University 2Center for Aging and Developmental Biology, 3Department of Neurology, 4Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, NY 5Center for Infectious Disease Research and Vaccinology, South Dakota State University

ORF5 and ORF2 are the main structural genes carrying determinants of virulence of PRRSV - B Kwon*, IH Ansari, AK Pattnaik, FA Osorio Nebraska Center for Virology and Department of Veterinary and Biomedical Sciences, University of Nebraska-Lincoln

Serological marker candidates identified on structural and non-structural proteins of PRRSV - M de Lima1,2*, AK Pattnaik1, EF Flores2, FA Osorio1 1Nebraska Center for Virology and Dept of Veterinary and Biomedical Sciences, University of Nebraska 2Dept of Microbiology and Parasitology, Federal University of Santa Maria, RS, Brazil

Development of a broadly protective PRRS vaccine - GJ Tobin*, R Bushnell, PL Nara Biological Mimetics, Inc., Frederick, MD

Expression of recombinant GP5 in transgenic corn callus for PRRSV va ccine development - R Lillie1, XJ Meng2, P Halbur3, C Zhang1* 1Department of Biological Systems Engineering, 2Department of Department of Biomedical Sciences and Pathobiology, Virginia Tech 3Dept of Veterinary Diagnostic and Production Animal Medicine, Iowa State University

Involvement of cysteine residue(s) within the M protein of PRRSV in infectious virion production - IH Ansari, BJ Kwon, FA Osorio, AK Pattnaik Department of Veterinary and Biomedical Sciences and Nebraska Center for Virology, University of Nebraska-Lincoln

Deletions and heterologous gene exression in nsp2 of PRRSV - D-Y Kim1, K-O Chang1, JG Calvert2, RRR Rowland1 1Dept of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University 2Pfizer Animal Health, Kalamazoo, MI

Immunization of swine with replicon particles: Proof of concept - MM Erdman1*, BG Crabtree1, KI Kamrud2, DL Harris 1,3,4, JF Smith2 1Dept of Animal Science 3 Veterinary Diagnostic and Production Animal Medicine, Iowa State University 2AlphaVa x Inc, Research Triangle Park, NC 4Sirrah LLC, Ames, IA

Assessment of the efficacy of commercial PRRSV vaccines based on measurement of serologic response, frequency of gamma-IFN producing cells and virological parameters of protection upon challenge - F Zuckermann1, E Alvarez Garcia2, I Diaz Luque3, J Christopher-Hennings4, A Doster5, M Brito5, F Osorio5 1Department of Veterinary Pathobiology, School of Vet Medicine, University of Illinois 2Depto de Sanidad Animal, Fac de Ciencias Veterinarias Univ Complutense de Madrid, Spain 3Depto de Sanitat i Anatomia Animals, Fac de Veterinària, Univ Autònoma de Barcelona, Bellaterra, Spain 4Dept of Veterinary Sciences, South Dakota State University 5Nebraska Center for Virology and Department of Veterinary and Biomedical Sciences, University of Nebraska-Lincoln

SECTION 5: ECOLOGY, EPIDEMIOLOGY, ELIMINATION
Aerosol transmission of PRRSV: An application to the field (preliminary data) - AN Pitkin1*, R Moon2, SA Dee1 1 Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota 2Department of Entomology, University of Minnesota

Susceptibility of young pigs to PRRSV infection by aerosol route of exposure - JR Hermann1, CA Muñoz-Zanzi2, K-J Yoon1, J Zimmerman1 1College of Veterinary Medicine, Iowa State University 2College of Veterinary Medicine, University of Minnesota

Effect of differences in route and time of exposure to PRRSV on the reproductive outcome of sows - K Vashisht*, R Husmann, LD Firkins, FA Zuckermann, TL Goldberg Dept of Pathobiology, College of Vet Medicine, University of Illinois

Novel antivirals against PRRSV - D Patel1* GH Edwards1, DA Stein2, PL Iversen2, Y Zhang1 1College of Veterinary Medicine, University of Maryland, College Park, MD 2AVI BioPharma Inc., Corvallis, OR

Production of PRRSV negative pigs from a PRRSV positive herd does not depend on serostatus of sows - D Nilubol*, S Nantawan Na Ayudhaya Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand

Regional Market Swine PRRSV Antibody Survey - JD McKean*, AM O'Connor, J Beary Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University

A Bayesian approach to evaluating test performance by combining information from multiple experts and data from a commercial ELISA - R Evans*, J Prickett, J Zimmerman College of Veterinary Medicine, Iowa State University

Serological prevalence of PRRSV in the Shandong area in China - J Wu1,2,3 , Y Liu2, X Zhang2, Z Niu3, J Wang2, D Yoo1 1Department of Pathobiology, Ontario Veterinary College, University of Guelph 2Shandong Academy of Agricultural Sciences, Jinan, Shandong, China 3Shandong Agricultural University, Tai’an, Shandong, China

Eradication of PRRSV In Chile - A Estrada, M Rojas, L Cuevas, H Rojas Servicio Agrícola y Ganadero, Santiago, Chile

Comparison of serum and meat juice antibody levels in experimental and field-derived swine - JD McKean Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University

Factors influencing viral disease transmission and elimination - S Mortensen The Danish Veterinary and Food Administration, Soeborg, Denmark

Environmental stability of infectious PRRSV - AC Jacobs1, JR Hermann1, C Muñoz-Zanzi2, MB Roof3, JJ Zimmerman1 1College of Veterinary Medicine, Iowa State University 2School of Public Health, University of Minnesota 3Boehringer Ingelheim Vetmedica, Inc., Ames, IA

Regional eradication of PRRSV – a pilot project - RB Morrison*, E Mondaca, S Wayne, SA Dee, P

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