Detection of <em>Actinobacillus pleuropneumoniae</em> in Oral Fluid Samples
Oral fluid testing has the potential to be a screening tool for detection of swine bacterial pathogens, Actinobacillus pleuropneumoniae, Haemophilus parasuis and Streptococcus suis, according to new research from the University of Minnesota.The use of cotton ropes has been recently
proposed to collect oral fluids from pigs as
a method to detect and monitor viral infections
in swine populations, according to Dr Giordana Costa and colleagues at the University of Minnesota in the US. In a paper published recently in Journal of Swine Health and Production, they explain that its use
for detection and monitoring of swine bacterial
pathogens by polymerase chain reaction
(PCR) has not been assessed.
In this study,
oral-fluid testing for diagnosis of Actinobacillus
pleuropneumoniae was evaluated over
time, utilising samples from experimentally
infected pigs.
Eighty pigs were randomly
assigned to experimental groups infected
with A. pleuropneumoniae serovars 1, 3, 5,
7, 10, 12 or 15 and a non-inoculated control
group. Oral fluids and blood samples
were collected prior to infection, one day
post–infection, and weekly thereafter for seven
consecutive weeks. Oral fluids were tested
for A. pleuropneumoniae, Haemophilus parasuis
and Streptococcus suis by specific PCR
tests offered by the Minnesota Veterinary
Diagnostic Laboratory.
A. pleuropneumoniae
was detected on days 1 and 7
post–infection, whereas H. parasuis and S. suis,
normal colonisers of the swine respiratory
tract, were detected at all time points.
Costa and co–authors report that their results indicate that oral-fluid testing has the
potential to be a screening tool for detection
of swine bacterial pathogens. Field studies
are indicated to explore this potential
further.
Reference
Costa G., S. Oliveira and J. Torrison. 2012. Detection of Actinobacillus pleuropneumoniae in oral-fluid samples obtained from experimentally infected pigs. J Swine Health Prod. 20(2):78–81.
Further Reading
![]() | - | You can view the full report by clicking here. |
Further Reading
![]() | - | Find out more information on the diseases mentioned in this article by clicking here. |
April 2012