Click on the link at the end to access the full proceedings and individual abstracts.

• EUROPRRS: Still paving the way? - T. Ait-Ali. p30
• Fostera™ PRRS: Modulation of interferon-alpha and induction of protective immunity two weeks following vaccination - J.G. Calvert et al. p31
• Porcine Reproductive and Respiratory Syndrome (PRRSV) Diagnostics in the United States: Past, Present and Future - J. Christopher-Hennings. p32
• Vaccine vectors based on Coronavirus genomes to protect against PRRSV and SARS-CoV - L. Enjuanes et al. p33
• The PRRSV replicase: structure, function and implications - Y. Fang et al. p34
• Principles of controlling PCV2 – the importance of controlling viremia - M.L. Linatoc p35
• Reflection of sow serology in piglet litters oral fluids – a sow farm surveillance field study - C.K. Goodell et al. p36
• Genetics of host resistance to PRRSV infection: Progress of the PRRS Host Genetics Consortium - J.K. Lunney et al. p37
• An update on PRRS immunology - E. Mateu. p38
• Breeding for PRRS resistance - G. McLaren and N. Deeb. p39
• Control of PRRS on farm and regional level - R. Morrison. p40
• Global Research on PRRS – development of STAR-IDAZ PRRS network - A. Morrow. p41
• Immunological interactions of PRRSV and pigs - M.P. Murtaugh et al. p42
• The pathogenesis of porcine reproductive and respiratory syndrome (PRRS) is fully determined by the intriguing interplay of PRRSV with monocytic cells - H. Nauwynck et al. p43
• Luminex® application for PCV2 and PRRSV diagnosis - T. Opriessnig. p44
• The role of host genetics in vaccine development - R.R.R. Rowland and B. Trible. p45
• Molecular evolution of PRRSV in Europe - T. Stadejek. p46
• Development and application of a dual-marker vaccine for highly pathogenic Porcine Reproductive and Respiratory Syndrome - G.Z. Tong et al. p47
• Porcine reproductive and respiratory syndrome situation in China: the past, present, and future - H. Yang. p48
• Scale-up and transformation of Chinese swine production in the next decade and analysis of high-efficacy hog production strategies - G. Yi. p49
• Structure and function of PRRSV proteins - D. Yoo et al. p50
• Towards a better PRRS vaccine: what are the missing links? Z. Wei et al. p51

Epidemiology and Disease Control

• Feed addition with Tilmicosin reduce PRRSV viral loads in pigs in vivo - M.T. Chiou et al. p52
• Operating system for gene surveillance of porcine reproductive and respiratory syndrome virus (PRRSV) in Korea - E.J. Choi et al. p53
• Development of Real Time RTPCR (RRT-PCR) commercial kit for reliable detection and typing of PPRSV - E. Redondo et al. p54
• PRRSV elimination in a 600 sow one-site system in China - Z.G. Liang et al. p55
• Evidence that Torque teno sus virus 1 (TTSuV1) is not associated with porcine circovirus associated diseases (PCVAD) - Y.W. Huang et al. p56
• The establishment of the PRRS detection liquid blocking ELISA method - H. Liu et al. p57
• Seroprevalence of PRRSV, swine influenza (SIV), and PCV2 in feral pigs - M.A. Kerrigan et al. p58
• Protective effects against PRRSV infection and boosting effects on PRRSV vaccine of immunostimulator (Barodon®) in pigs - C.H. Kwon et al. p59
• Application of high molecular Poly-gamma-glutamic acid on Pigs infected with Porcine Reproductive and Respiratory Syndrome virus - J.H. Lee et al. p60
• Genetic analysis and phylogenetic tree of porcine circovirus type 2 field strains isolated from slaughtered pigs in Korea - M.I. Lee et al. p61
• Recombination leads to the reemergence of highly pathogenic PRRSV in China - M. Shi and F.C. Leung. p62
• Comparison of viremia of type II porcine reproductive and respiratory syndrome virus in natural PRRSV infected pigs by zip nucleic acid probes based real-time PCR - C.N. Lin et al. p63
• Comparison and application of several porcine circovirus type 2 antibody detection kits - Z.H. Zhang et al. p64
• Prevalence of porcine hokovirus and its co-infection with porcine circovirus 2 in China - S.B. Li et al. p65
• Three new emerging subgroups of torque teno sus virus (TTSuVs) and co-infection of TTSuV with porcine circovirus type 2 in China - J.B. Liu et al. p66
• Quantification of PRRSV transmission: effect of pig vaccination - E. Pileri et al. p67
• The isolation and identification of new subtype of PCV2a - Q. Xiaohuo et al. p68
• Development of best practices to diagnose Porcine Reproductive and Respiratory Syndrome (PRRS) virus subtypes with high sensitivity in swine - R. Rauh et al. p69
• Discrimination of Porcine Reproductive and Respiratory Syndrome virus type I and type II by an indirect ELISA - M. Frias et al. p70
• Detection of porcine reproductive and respiratory syndrome virus (PRRSV) using the light scattering assay (LSA) based on the microfluidic chip - J.Y. Song et al. p71
• Serosurvey and molecular detection of PRRSV-1 in European wild boars (Sus scrofa) in Lithuania - A. Stankevicius et al. p72
• Genetic analysis of porcine reproductive and respiratory syndrome virus strains in Japan from 1992 to 2011 - M. Takagi et al. p73
• Vaccination against PRRS and PCV2 reduced the loss in nursery: a case report - D. Xu et al. p74
• PRRSV and other co-infecting viruses in PMWS affected pigs - S. Vilcek et al. p75
• The first detection of PRRSV in wild boars hunted in Central European region, Slovakia - S. Vilcek et al. p76
• Porcine Reproductive and Respiratory Syndrome in hybrid wild boars, China - J.Q. Wu et al. p77
• Exploration of large breeding herd with PRRSV serum acclimation - S.Q. Wu et al. p78
• A potential novel Porcine Reproductive and Respiratory Syndrome virus subgroup emerged in south China based on the genetic analysis of the ORF5 gene from 2007 to 2011 - J.X. Xie et al. p79
• Study on antiviral activity of four Chinese herbs to Porcine Reproductive and Respiratory Syndrome virus. J.J. He et al. p80
• Effects of ozone, ultraviolet and disinfectant against Porcine Reproductive and Respiratory Syndrome virus - Y.D. Yoon and W.I. Kim. p81
• Preparation and immune activities of specific transfer factor against porcine reproductive and respiratory syndrome - H.X. Yuan et al. p82
• Possible cross-species infection of porcine circoviruses and its control measures - S.L. Zhai et al. p83
• Preparation of monoclonal antibody against PRRSV Nsp9 gene and establishment of block-ELISA method - Z.Q. Huang et al. p 84
• The Analysis of Genetic Variation among 19 Nsp2 Genes of PRRSV Isolated from Southwestern China in 2009-2012 - Y.S. Zhou et al. p85

Genetics

• Autophagy is involved in genetic resistance to PRRSV infection - B. Ding et al. p86
• Cloning and functional analysis of 5′regulatory region of porcine CYP3A88 gene - C.L. Jiang et al. p87
• Generation of an infectious clone of SDA2, a highly virulent North American-type isolate of porcine reproductive and respiratory syndrome virus - C.L. Jiang et al. p88
• Cloning and functional analysis of 5′regulatory region of porcine USP18 gene - Y.P. Li et al. p89
• Porcine circovirus type 2 (PCV2): genetic variation and newly emerging genotypes in China - L.J. Guo et al. p90
• Analysis of synonymous codon usage in Porcine Reproductive and Respiratory Syndrome virus - Y.S. Liu et al. p91
• Antiviral activities of the porcine IFIT family genes against PRRSV - X. Zhou et al. p92

Vaccines and Immunity

• PRRS modified live vaccine adjuvanted with Montanide™ Gel01 ST provides enhanced protection against homologous PRRSV infection in swine - A.J. Galliher-Beckley et al. p93
• Distribution of PRRSV and PCV2 in sera and tissues and pulmonary findings after PCV2 vaccination in field pigs - C.C. Chang et al. p94
• The effects on distribution of pathogens, reproductive performance of sows and growth performance of weaned pigs after attenuated live PRRSV vaccination in sows - C.C. Chang and L.C. Yang. p95
• Evaluation of a DNA vaccine candidate co-expressing GP3 and GP5 of PRRSV with interferon alpha/gamma in immediate and long-lasting protection against highly pathogenic PRRSV challenge - Y.J. Du et al. p96
• The immunization of pigs with a Gp3 subunit vaccine elicits specific antibody response but does not protect against PRRSV infection after challenge - M. García Durán et al. p97
• Development and validation of porcine reproductive and respiratory syndrome virus specific neutralizing antibody detection assay in pig oral fluid samples - K. Ouyang et al. p98
• Characterisation of the T cell response induced by infection with subtype 1 or 3 European porcine reproductive and respiratory syndrome viruses - H. Mokhtar et al. p99
• Development of a multiplex fluorescent microsphere immunoassay for diagnosis of porcine respiratory disease complex - S.R. Lawson et al. p100
• Evaluation of the efficacy of an attenuated live vaccine against highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) in young pigs - X. Leng et al. p101
• Virulence evaluation of a modified live highly pathogenic porcine reproductive and respiratory syndrome virus vaccine by serial passages in pigs - X. Leng et al. p102
• Changes of different lymphocyte contents in peripheral blood from pigs co-infected with porcine circovirus type 2 and porcine parvovirus - H.Y. Li et al. p103
• Peptide nanofiber hydrogel: A novel adjuvant for Porcine Reproductive and Respiratory Syndrome virus modified live vaccine - X. Li et al. p104
• Attenuation of interferon antagonizing function of PRRSV by targeted mutations in a highly conserved motif of nsp1beta protein - Y.H. Li et al. p105
• Comparative respiratory pathogenicity and dynamic distribution of highly pathogenic porcine reproductive and respiratory syndrome virus and its attenuated vaccine strain in piglets - C. Liu. p106
• Construction and biological characteristics of a recombinant porcine circovirus type 2 expressing the VP1 neutralizing epitope of foot-and-mouth disease virus - F.Y. Zhang et al. p107
• Enhanced protective immune response to PCV2 subunit vaccine by co-administration of recombinant porcine IFN-gamma in mice - Y.P. Wang et al. p108
• Immunity induced by an experimental vaccine based on the recombinant PRRSV GP5 protein without ADE epitope expressed in adenovirus in pigs - M. Yang et al. p109
• Modulation of swine interleukin-4 to induce virus-specific immune responses elicited by modified live porcine reproductive and respiratory syndrome virus (PRRSV) vaccine - J. Peng et al. p110
• Construction and immunogenicity of DNA vaccine coexpressing GP3 and GP5 proteins of European-type Porcine Reproductive and Respiratory Syndrome virus - J.Q. Ren et al. p111
• Inhibition of porcine circovirus type 2 replication by cap protein gene DNA vaccine in mice - S. Sylla et al. p112
• Preliminary study of recombinant Bordetella Bronchiseptica expressing cap protein of Porcine Circovirus type 2 as a candidate vaccine against porcine Circovirus disease - S. Jin et al. p113
• Isolation of the European genotype porcine reproductive and respiratory syndrome virus (PRRSV) in Kazakhstan - K.K. Tabynov et al. p114
• Effect of vaccination with Ingelvac Circoflex® on fattening performance in a farm in north China - A. Wang et al. p115
• Construction of recombinant PRRSV expressing granulocytemacrophage colony-stimulating factor - L.X. Yu et al. p116
• Evidence for a broadly neutralizing antibody response to PRRSV - B.R. Trible et al. p117
• Host recognition of the structural form of the PCV2 capsid protein results in protective immunity or immunopathogenesis - B.R. Trible and R.R.R. Rowland. p118
• The comparison of immune efficacy between combined PCV2, MH vaccine inactivated and the corresponding single vaccines - G.H. Wang et al. p119
• Identification of a B-cell epitope in the glycosylated protein 3 of highly pathogenic porcine reproductive and respiratory syndrome virus HuN4 strain - Y. Sun et al. p120
• Anti-idiotypic antibody vaccine against highly pathogenic PRRSV infection - C. Zhang et al. p121
• The nonstructural protein 1 papain-like cysteine protease was not necessary for porcine reproductive and respiratory syndrome virus nonstructural protein 1 to inhibit interferon-beta function - X.B. Shi et al. p122
• Toll-like receptor ligands enhance the protective effects of vaccination against porcine reproductive and respiratory syndrome virus in swine - L.L. Zhang et al. p123
• The changes of the lymphocyte subpopulations in peripheral blood of the pigs inoculated with highly pathogenic PRRSV GD wild and vaccine type - W.L. Zhang et al. p124

Viral Heterogeneity and Evolution

• Investigation of recombination in Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) - R.J. Chand et al. p125
• A novel isolate with deletion in GP3 gene of Porcine Reproductive and Respiratory Syndrome virus from the mideast of China - B.C. Fan et al. p126
• swIFN-beta promotes genetic mutation of porcine reproductive and respiratory syndrome virus in Marc-145 - D.S. He et al. p127
• Experimental infection of pigs with two east European variants of Type 1 PRRSV - C.K. Hjulsage et al. p128
• Fast and robust methods for full genome sequencing of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Type 1 and Type 2 - L.K. Kvisgaard et al. p129
• The diversity of Porcine Reproductive and Respiratory Syndrome virus type 1 and 2 in Denmark - L.K. Kvisgaard et al. p130
• The evidence of porcine respiratory and reproductive syndrome virus evolved under pressure from deaminases - C.L. Leng et al. p131
• Genomic sequencing and genetic variation analysis of highly pathogenic porcine reproductive and respiratory syndrome virus - C. Liu et al. p132
• Recombination events are common in genotype 1 and 2 PRRS virus worldwide and may generate mosaic viruses - G.E. Martin-Valls et al. p133
• Evolution dynamics of highly pathogenic type 2 PRRSV: analysis of envelope protein coding genes - V.G. Nguyen et al. p134
• Phylogentic analysis and molecular characteristics of five variant Chinese field isolates of PRRSV - Z.F. Pei et al. p136
• A cell culture-derived deletion in the nsp2 region of a North American PRRSV overlaps with a unique nsp2 deletion reported in a Chinese PRRSV isolate - R. Ransburgh et al. p137
• A novel strain of PCV2 isolated from vaccinated pigs suffering PCVAD causes 40% mortality in CDCD pigs in the presence of PPV - A.W. Suddith and R. Hesse. p138
• A monoclonal antibody against the N of a HP-PRRS vaccine strain(HuN4-F112) could not recognize its virulent strain (HuN4-F5) not due to their amino acid sequence difference - Q. Wang et al. p139
• Molecular basis associated with the pathogenicity of a genotype 1 PRRSV isolate - X.C. Wang et al. p140
• Phylogenetic analysis and molecular characteristics of 17 porcine reproductive and respiratory syndrome virus isolates in South China from 2010-2011 - M.Z. Zhang et al. p141

Virus Replication-Protein Structure-Function

• Non-structural protein 1-mediated interferon modulation is a common strategy for immune evasion in arteriviruses - M. Han et al. p142
• Suppression of type I interferon production mediated by PRRS virus non-structural protein 1-beta - M. Han et al. p143
• Construction and biological characterization of recombinant porcine circovirus type 2 expressing the V5 epitope tag - L.P. Huang et al. p144
• Identification of one critical amino acid that determines a conformational neutralizing epitope in the capsid protein of porcine circovirus type 2 - L.P. Huang et al. p145
• Mutation in a conserved motif of porcine circovirus type 2 capsid protein reduces capsid protein expression, hampers colocalization between capsid and replication proteins and impacts viral fitness - L.P. Huang et al. p146
• Identification of porcine reproductive and respiratory syndrome virus ORF1a-encoded replicase proteins in virusinfected cells - Y.H. Li et al. p147
• Antigenic epitope mapping using the monoclonal antibody against the recombinant Rep’ protein of porcine circovirus type 2 - D. Liu et al. p148
• Characterization of two chimeric viruses by exchanging ORF2 gene of porcine circovirus type 1 (PCV1) and type 2 (PCV2) - C.M. Liu et al. p149
• Natural recombinant mutants, resulting from replacement of an open reading frame 2 (ORF2)-containing genomic fragment of porcine circovirus (PCV) genotype 2b with that of genotype 2a, showed significantly enhanced viral replication and altered antigenicity in vitro - L.J. Guo et al. p150
• Discovery and characterization of the involvement of a cellular membrane fusion protein in PRRSV replication - J.A. Hicks et al. p151
• Amino acid mutations in the capsid protein produce novel porcine circovirus type 2 neutralizing epitopes - J.B. Liu et al. p152
• RNA interference targeting nucleocapsid protein (N) inhibits porcine reproductive and respiratory syndrome virus replication in Marc-145 cells - M. Yang et al. p153
• Functional analysis of ORF4 protein encoded by porcine circovirus type 2 - N. Zhou et al. p154
• ORF4 protein: a newly discovered virus protein in PCV2 replication - J.L. He et al. p155
• Co-infection study between porcine reproductive and respiratory syndrome virus and a bacteria led to the discovery of a possible new bacteria antiviral - C. Provost et al. p156
• Impact of co-infection by porcine circovirus (PCV) and porcine reproductive and respiratory syndrome virus (PRRSV) in porcine pulmonary epithelial cell line - C. Provost et al. p157
• The correlation of cyclin A expression level with porcine circovirus type 2 propagation efficiency - Q.H. Tang et al. p158
• Dissection for the structure and function of a 5'-proximal prominent stem-loop in Porcine Reproductive and Respiratory Syndrome virus - F. Gao et al. p159
• Signal peptide cleavage from GP5 of PRRSV: Only a minor subfraction of molecules retains the decoy epitope, a presumed molecular cause for viral persistence - B. Thaa et al. p160
• Non-structural protein 2 of the porcine reproductive and respiratory syndrome (PRRS) virus: a crucial protein in viral pathogenesis, immunity and diagnosis - F.X. Wang et al. p161
• Role of non-structural protein 2 in the regulation of the replication of the porcine reproductive and respiratory syndrome virus in MARC-145 cells: effect of gene silencing and over expression - F.X. Wang et al. p162
• The effect of the position of N-linked glycosylation sites within hypervariable region of GP5 on virus neutralization - Z.Z. Wei et al. p163
• The role of antibody in PRRSV infection PAM - Q.Y. Yang et al. p164
• Regulation of host cell gene expression and cell cycle progression by nsp11 of PRRS virus - Y. Sun et al. p165
• Concurrent highly pathogenic porcine reproductive and respiratory syndrome virus infection accelerates Haemophilus parasuis infection in conventional pigs - J. Yu et al. p166
• Mutagenesis analysis of porcine reproductive and respiratory syndrome virus nonstructural protein 7 - M.Z. Zhang et al. p167

Virus-Host Interaction

• The host miR-26a inhibits porcine reproductive and respiratory syndrome virus replication by activating innate antiviral immunity - C.W. Chen et al. p168
• The application of deep sequencing for the analysis of mutations within hypervariable regions of PRRSV - N. Chen et al. p169
• The effect of knockdown of Nonmuscle myosin II-A on Porcine Reproductive and Respiratory Syndrome Virus infection - J. Gao et al. p170
• MicroRNA-181 suppresses PRRSV infection by targeting its receptor CD163 - L. Gao et al. p171
• Nonstructural proteins 1beta and 11 play an important role in differential TNF-alpha production-induced by different PRRSV strains - Q. He et al. p172
• Using high throughput proteomics to understand the functional interaction of PRRSV and the nucleocapsid protein with the host cell and comparison with the coronavirus infectious bronchitis virus - H.Y. Chen et al. p173
• Fetal preservation and virus concentration following type 2 PRRSV infection in pregnant gilts - A. Ladinig et al. p174
• PRRS RNA concentration in serum does not predict clinical severity in pregnant gilts - A. Ladinig et al. p175
• Temporal changes in white blood cell counts and PBMC subsets following type 2 PRRSV in pregnant gilts - A. Ladinig et al. p176
• A porcine circovirus type 2 (PCV2) mutant with 234 amino acids in capsid protein showed more virulence in vivo, compared with classical PCV2a/b strains. L.J. Guo et al. p177
• The role of Hsp70 in the replication of porcine circovirus type 2 in vitro - J. Liu et al. p178
• Porcine reproductive and respiratory syndrome virus (PRRSV)-mediated NF-κB activation is dependent on TLR2, TLR4 and MAPK signaling pathway - R.Luo et al. p179
• Quantitative mitochondrial proteomics by isobaric tags for relative and absolute quantification (iTRAQ) labeling in PRRSV infected pulmonary alveolar macrophages - R. Luo et al. p180
• PRRSV infection change Bax/Bcl-2 ratio in primary porcine alveolar macrophages - Y. Mu et al. p181
• Porcine, murine and human sialoadhesin: portals for PRRSV entry into target cells - W. Van Breedam et al. p182
• PRRSV infection induces Sn expression on local endometrial and placental CD163-positive cells - U.U. Karniychuk et al. p183
• Phenotypic modulation and cytokine profiles of antigen presenting cells by European subtype 1 and 3 porcine reproductive and respiratory syndrome virus strains in vitro and in vivo - E. Weesendorp et al. p184
• Genome-wide analysis of marker genes related to antiviral regulation in PRRSV-infected porcine macrophages at different activation statuses - Y. Sang et al. p185
• In vivo impact of deoxynivalenol naturally contaminated feed on porcine reproductive and respiratory virus infection - C. Savard et al. p186
• The susceptibility of porcine myeloid cells to Porcine Reproductive and Respiratory Syndrome virus - H. Singleton et al. p187
• PRRSV type 1 can induce aggravation of infection with a mild virulent Actinobacillus serotype 2 strain - N. Stockhofe-Zurwieden et al. p188
• Identification of differentially expressed proteins in porcine alveolar macrophages infected with virulent/attenuated strains of porcine reproductive and respiratory syndrome virus - J.P. Zhu et al. p189
• Porcine SAMHD1 blocks HPPRRSV replication in Marc-145 cells - S. Yang et al. p190
• Reduction of PRRSV infection in Marc-145 cells that express the exogenous C-terminal domain of non-muscle myosin heavy chain II-A - R. Wei et al. p191
• Experimental infection and comparative genomic analysis of a highly pathogenic PRRSV-HBR strain at different passage levels - Y.W. Wei et al. p192
• Synergistic effects of sequential infection with highly pathogenic porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 in piglets - P.H. Fan et al. p193
• Swine immunoglobulin lambda-like polypeptide 5, an adaptor of RIG-1 and MDA5, regulates type I Interferon production and PRRSV replication through interaction with PRRSV non-structural proteins - Q.F. Liu et al. p194
• Heme oxygenase 1 inhibition of HP-PRRSV infection in Marc-145 cell lines - A. Zhang et al. p195
• USP18 restricts PRRSV growth through alteration of nuclear translocation of NF-κB p65 and p50 in MARC-145 cells - D. Xu et al. p196
• Induction of ROS generation and NF-kappa B activation in MARC-145 cells by a novel Porcine Reproductive and Respiratory Syndrome virus isolated in the southwest of China - Y. Yan et al. p197
• Nonstructural proteins 4 and 10 of highly pathogenic PRRSV induce apoptosis in virus-infected MARC-145 cells - S.Z. Yuan et al. p198
• Porcine reproductive and respiratory syndrome virus activates inflammasomes of porcine alveolar macrophages via its small envelope protein E - K. Zhang et al. p199
• Non-muscle myosin heavy chain II-A is a novel cellular receptor for PRRSV - J. Gao et al. p200

September 2014