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5.1.3 Immunoperoxidase monolayer assay (IPMA) for the detection of antibodies against Lawsonia intracellularis

The immunoperoxidase monolayer assay (IPMA) is similar to the IFA. The anti species secondary antibody is labelled with peroxidase, which leads to brown staining by a substrate that can be evaluated using a light microscope, rather than fluorescent labels as with the IFA, resulting in fewer false positive results.

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